| RRC ID |
6542
|
| 著者 |
Hu W, Abe-Dohmae S, Tsujita M, Iwamoto N, Ogikubo O, Otsuka T, Kumon Y, Yokoyama S.
|
| タイトル |
Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo.
|
| ジャーナル |
J Lipid Res
|
| Abstract |
Serum amyloid A (SAA) was markedly increased in the plasma and in the liver upon acute inflammation induced by intraperitoneal injection of lipopolysaccharide (LPS) in mice, and SAA in the plasma was exclusively associated with HDL. In contrast, no HDL was present in the plasma and only a small amount of SAA was found in the VLDL/LDL fraction (d < 1.063 g/ml) after the induction of inflammation in ABCA1-knockout (KO) mice, although SAA increased in the liver. Primary hepatocytes isolated from LPS-treated wild-type (WT) and ABCA1-KO mice both secreted SAA into the medium. SAA secreted from WT hepatocytes was associated with HDL, whereas SAA from ABCA1-KO hepatocytes was recovered in the fraction that was >1.21 g/ml. The behavior of apolipoprotein A-I (apoA-I) was the same as that of SAA in HDL biogenesis by WT and ABCA1-KO mouse hepatocytes. Lipid-free SAA and apoA-I both stabilized ABCA1 and caused cellular lipid release in WT mouse-derived fibroblasts, but not in ABCA1-KO mouse-derived fibroblasts, in vitro when added exogenously. We conclude that both SAA and apoA-I generate HDL largely in hepatocytes only in the presence of ABCA1, likely being secreted in a lipid-free form to interact with cellular ABCA1. In the absence of ABCA1, nonlipidated SAA is seemingly removed rapidly from the extracellular space.
|
| 巻・号 |
49(2)
|
| ページ |
386-93
|
| 公開日 |
2008-2-1
|
| DOI |
10.1194/jlr.M700402-JLR200
|
| PII |
S0022-2275(20)42859-7
|
| PMID |
18033752
|
| MeSH |
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters / biosynthesis
ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / physiology*
Animals
Apolipoprotein A-I / biosynthesis
BALB 3T3 Cells
CHO Cells
Chromatography, Gel
Chromatography, High Pressure Liquid
Cricetinae
Cricetulus
Female
Gene Expression Regulation / physiology
Hepatocytes / metabolism
Hepatocytes / physiology
Lipoproteins, HDL / biosynthesis*
Lipoproteins, HDL / blood
Lipoproteins, HDL / genetics
Liver / cytology
Liver / metabolism
Liver / physiology*
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Knockout
Serum Amyloid A Protein / physiology*
|
| IF |
4.483
|
| 引用数 |
33
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
BALB/3T3
CHO-K1(RCB0285) |
