RRC ID 65575
Author Shimizu Y, Ito Y, Tanaka H, Ohshima T.
Title Radial glial cell-specific ablation in the adult Zebrafish brain.
Journal Genesis
Abstract The zebrafish brain can continue to produce new neurons in widespread neurogenic brain regions throughout life. In contrast, neurogenesis in the adult mammalian brain is restricted to the subventricular zone (SVZ) and dentate gyrus (DG). In neurogenic regions in the adult brain, radial glial cells (RGCs) are considered to function as neural stem cells (NSCs). We generated a Tg(gfap:Gal4FF) transgenic zebrafish line, which enabled us to express specific genes in RGCs. To study the function of RGCs in neurogenesis in the adult zebrafish brain, we also generated a Tg(gfap: Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, which allowed us to induce cell death exclusively within RGCs upon addition of metronidazole (Mtz) to the media. RGCs expressing nitroreductase were specifically ablated by the Mtz treatment, decreasing the number of proliferative RGCs. Using the Tg(gfap:Gal4FF; UAS:nfsB-mcherry) transgenic zebrafish line, we found that RGCs were specifically ablated in the adult zebrafish telencephalon. The Tg(gfap:Gal4FF) line could be useful to study the function of RGCs.
Volume 53(7)
Pages 431-9
Published 2015-7-1
DOI 10.1002/dvg.22865
PMID 26045148
MeSH Ablation Techniques / methods Animals Animals, Genetically Modified Brain / cytology* Brain / metabolism Dentate Gyrus / cytology Dentate Gyrus / metabolism Ependymoglial Cells / cytology* Ependymoglial Cells / metabolism Glial Fibrillary Acidic Protein / biosynthesis Glial Fibrillary Acidic Protein / genetics Neural Stem Cells / cytology Neural Stem Cells / metabolism Neurogenesis / physiology Zebrafish
IF 1.76
Zebrafish RIKEN WT Tg(UAS:nfsB-mCherry)rw0144