RRC ID 65579
著者 Takeuchi M, Yamaguchi S, Sakakibara Y, Hayashi T, Matsuda K, Hara Y, Tanegashima C, Shimizu T, Kuraku S, Hibi M.
タイトル Gene expression profiling of granule cells and Purkinje cells in the zebrafish cerebellum.
ジャーナル J Comp Neurol
Abstract The structure of the neural circuitry of the cerebellum, which functions in some types of motor learning and coordination, is generally conserved among vertebrates. However, some cerebellar features are species specific. It is not clear which genes are involved in forming these conserved and species-specific structures and functions. This study uses zebrafish transgenic larvae expressing fluorescent proteins in granule cells, Purkinje cells, or other cerebellar neurons and glial cells to isolate each type of cerebellar cells by fluorescence-activated cell sorting and to profile their gene expressions by RNA sequencing and in situ hybridization. We identify genes that are upregulated in granule cells or Purkinje cells, including many genes that are also expressed in mammalian cerebella. Comparison of the transcriptomes in granule cells and Purkinje cells in zebrafish larvae reveals that more developmental genes are expressed in granule cells, whereas more neuronal-function genes are expressed in Purkinje cells. We show that some genes that are upregulated in granule cells or Purkinje cells are also expressed in the cerebellum-like structures. Our data provide a platform for understanding the development and function of the cerebellar neural circuits in zebrafish and the evolution of cerebellar circuits in vertebrates. J. Comp. Neurol. 525:1558-1585, 2017. © 2016 Wiley Periodicals, Inc.
巻・号 525(7)
ページ 1558-1585
公開日 2017-5-1
DOI 10.1002/cne.24114
PMID 27615194
MeSH Animals Animals, Genetically Modified Cerebellum / cytology* Cerebellum / embryology Cerebellum / growth & development Flow Cytometry Gene Expression Profiling Immunohistochemistry In Situ Hybridization Microarray Analysis Neurogenesis / genetics* Neurons / cytology* Polymerase Chain Reaction Purkinje Cells / cytology* Transcriptome* Zebrafish* / embryology Zebrafish* / genetics Zebrafish* / growth & development
IF 2.801
リソース情報
ゼブラフィッシュ aldoca:gap43-Venus aldoca:gap43-Venus gSA2AzGFF152B hspzGFFgDMC156A hspGFFDMC28C SAGFF(LF)251A UAS:GFP