Reference - Detail
| RRC ID | 65620 |
|---|---|
| Author | Alharthy SA, Hardej D. |
| Title | The role of transcription factor Nrf2 in the toxicity of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) in C57BL/6 mouse astrocytes. |
| Journal | Environ Toxicol Pharmacol |
| Abstract |
Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are members of perfluoroalkyl substances (PFAS). This study aimed to determine the protective role of Nrf2 against the toxicity of these agents. Nrf2-/- and wild-type astrocytes were exposed to PFOS (75-600 μM) and PFOA (400-1000 μM) for 24 h. Lactate dehydrogenase (LDH) release was significantly higher in nrf2-/- than in the wild-type astrocytes. Exposure to 600 μM PFOS and 800 μM PFOA showed significant increases in reactive oxygen species, lipid peroxidation, and apoptosis in nrf2-/- astrocytes as compared to wild-type astrocytes. The GSH/GSSG ratio was significantly decreased in nrf2-/- astrocytes when compared to wild-type astrocytes. Additionally, PFOS and PFOS caused dramatic ultrastructural alterations to the mitochondria. BHT pretreatment in wild-type cells decreased ROS production with exposure to both agents. Results of the present study conclude that PFOS and PFOA are cytotoxic to astrocytes and that nrf2 -/- cells are more sensitive to toxicity by these agents. |
| Volume | 86 |
| Pages | 103652 |
| Published | 2021-8-1 |
| DOI | 10.1016/j.etap.2021.103652 |
| PII | S1382-6689(21)00070-3 |
| PMID | 33812015 |
| MeSH | Alkanesulfonic Acids / toxicity* Animals Apoptosis / drug effects Astrocytes / drug effects* Astrocytes / metabolism Caprylates / toxicity* Cells, Cultured Fluorocarbons / toxicity* L-Lactate Dehydrogenase / metabolism Mice, Inbred C57BL Mice, Knockout NF-E2-Related Factor 2 / genetics* Oxidative Stress / drug effects Reactive Oxygen Species / metabolism |
| IF | 3.292 |
| Resource | |
| Mice | RBRC01390 |