RRC ID 65726
著者 Park M, Yoon HJ, Kang MC, Kwon J, Lee HW.
タイトル MiR-338-5p enhances the radiosensitivity of esophageal squamous cell carcinoma by inducing apoptosis through targeting survivin.
ジャーナル Sci Rep
Abstract Radioresistance is a challenge in the treatment of esophageal squamous cell carcinoma (ESCC). MicroRNAs (miRNAs) are known to play an important role in the functional modification of cancer cells and recent studies have reported miRNA-mediated radiotherapy resistance. However, further research is necessary to reveal the regulation mechanisms, and treatment strategies using miRNA are yet to be established for ESCC. We compared the miRNA expression profiles of ESCC parental (TE-4) and acquired radioresistance (TE-4R) cell lines using a miRNA microarray and qRT-PCR. Our data showed that miR-338-5p, one of the target miRNA biomarkers, was significantly downregulated in TE-4R. Ectopic overexpression of miR-338-5p induced apoptosis and sensitivity to radiation treatment by interfering with survivin, which is a known inhibitor of apoptosis. Overexpression of survivin reversed miR-338-5p-induced apoptosis. Tumor xenograft experiments indicated that therapeutic delivery of the miR-338-5p mimics via direct injection into tumor mass increased sensitivity to radiation therapy. In conclusion, our findings suggest that miR-338-5p is a potential radiosensitizer and may be a therapeutic biomarker for radioresistant in ESCC.
巻・号 7(1)
ページ 10932
公開日 2017-9-7
DOI 10.1038/s41598-017-10977-9
PII 10.1038/s41598-017-10977-9
PMID 28883406
PMC PMC5589838
MeSH Animals Apoptosis* Disease Models, Animal Esophageal Neoplasms / pathology* Esophageal Neoplasms / radiotherapy Esophageal Squamous Cell Carcinoma / pathology* Esophageal Squamous Cell Carcinoma / radiotherapy Gene Expression Profiling Heterografts MicroRNAs / administration & dosage* MicroRNAs / metabolism* Neoplasm Transplantation Radiation Tolerance* Real-Time Polymerase Chain Reaction Survivin / antagonists & inhibitors* Treatment Outcome
IF 3.998
リソース情報
ヒト・動物細胞 TE-4(RCB2097) TE-14(RCB2101)