RRC ID |
6583
|
著者 |
Uekusa H, Namimatsu M, Hiwatashi Y, Akimoto T, Nishida T, Takahashi S, Takahashi Y.
|
タイトル |
Cadmium interferes with the degradation of ATF5 via a post-ubiquitination step of the proteasome degradation pathway.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
ATF5 is a member of the CREB/ATF family of transcription factors. In the current study, using a transient transfection system to express FLAG epitope fusion proteins of ATF5, we have shown that CdCl(2) or NaAsO(3) increases the protein levels of ATF5 in cells, and that cadmium stabilizes the ATF5 protein. Proteasome inhibitors had a similar effect to cadmium on the cellular accumulation of ATF5. Proteasome inhibition led to an increase in ubiquitinated ATF5, while cadmium did not appear to reduce the extent of ATF5 ubiquitination. ATF5 contains a putative nuclear export signal within its N-terminus. We demonstrated that whereas deletion of N-terminal region resulted in a increase of ATF5 levels, this region does not appear to be involved in the ubiquitination of ATF5. These results indicate that ATF5 is targeted for degradation by the ubiquitin-proteasome pathway, and that cadmium slows the rate of ATF5 degradation via a post-ubiquitination mechanism.
|
巻・号 |
380(3)
|
ページ |
673-8
|
公開日 |
2009-3-13
|
DOI |
10.1016/j.bbrc.2009.01.158
|
PII |
S0006-291X(09)00206-X
|
PMID |
19285020
|
MeSH |
Activating Transcription Factors / drug effects*
Activating Transcription Factors / genetics
Activating Transcription Factors / metabolism*
Animals
Arsenites / pharmacology
COS Cells
Cadmium Chloride / pharmacology*
Chlorocebus aethiops
Humans
Proteasome Endopeptidase Complex / metabolism*
Proteasome Inhibitors
Protein Stability / drug effects
Protein Structure, Tertiary
Ubiquitination*
|
IF |
2.985
|
引用数 |
12
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
COS-7(RCB0539) |