| RRC ID |
65843
|
| 著者 |
Igarashi T, Araki K, Yokobori T, Altan B, Yamanaka T, Ishii N, Tsukagoshi M, Watanabe A, Kubo N, Handa T, Hosouchi Y, Nishiyama M, Oyama T, Shirabe K, Kuwano H.
|
| タイトル |
Association of RAB5 overexpression in pancreatic cancer with cancer progression and poor prognosis via E-cadherin suppression.
|
| ジャーナル |
Oncotarget
|
| Abstract |
Pancreatic cancer is a common type of cancer with poor prognosis worldwide. Postoperative survival depends on the existence of metastasis. Elucidation of the mechanism underlying cancer progression is important to improve prognosis. The RAS-associated protein RAB5 activates intracellular membrane trafficking, and RAB5 expression is correlated to progression and epithelial mesenchymal transition in various cancers.The expression of RAB5 and E-cadherin in 111 pancreatic cancer samples was investigated by immunohistochemical staining, and the relationship among RAB5 expression, clinicopathological factors, and E-cadherin expression was assessed. Furthermore, RAB5 suppression analysis by siRNA was performed to determine the roles of RAB5 in morphological change, proliferation potency, cell migration ability, and invasiveness of the pancreatic cancer cell line.High RAB5 expression correlated with the presence of lymphatic invasion and venous invasion and low E-cadherin expression. Patients with high RAB5 expression had a poorer prognosis than those with low RAB5 expression. RAB5 suppression in pancreatic cancer cells enhanced E-cadherin expression; changed cell morphology from spindle to round; and inhibited proliferation, invasion, and cell migration.RAB5 contributes to poor prognosis and progression in pancreatic cancer patients. It may be a promising candidate for individualized therapy in refractory pancreatic cancer.
|
| 巻・号 |
8(7)
|
| ページ |
12290-12300
|
| 公開日 |
2017-2-14
|
| DOI |
10.18632/oncotarget.14703
|
| PII |
14703
|
| PMID |
28103577
|
| PMC |
PMC5355344
|
| MeSH |
Adult
Aged
Aged, 80 and over
Antigens, CD
Blotting, Western
Cadherins / biosynthesis*
Cadherins / genetics
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Disease Progression
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Male
Microscopy, Fluorescence
Middle Aged
Pancreas / metabolism*
Pancreas / pathology
Pancreatic Neoplasms / genetics
Pancreatic Neoplasms / metabolism*
Pancreatic Neoplasms / pathology
Prognosis
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
rab5 GTP-Binding Proteins / biosynthesis*
rab5 GTP-Binding Proteins / genetics
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
MIA Paca2(RCB2094)
PANC-1(RCB2095) |
