RRC ID 65871
Author Tanabe K, Shinsato Y, Furukawa T, Kita Y, Hatanaka K, Minami K, Kawahara K, Yamamoto M, Baba K, Mori S, Uchikado Y, Maemura K, Tanimoto A, Natsugoe S.
Title Filamin C promotes lymphatic invasion and lymphatic metastasis and increases cell motility by regulating Rho GTPase in esophageal squamous cell carcinoma.
Journal Oncotarget
Abstract To establish treatments to improve the prognosis of cancer patients, it is necessary to find new targets to control metastasis. We found that expression of FilaminC (FLNC), a member of the actin binding and cross-linking filamin protein family is correlated with lymphatic invasion and lymphatic metastasis in esophageal squamous cell carcinoma (ESCC) by increasing cell motility through activation of Rho GTPase.Immunohistochemistry analysis showed that FLNC expression in ESCC is associated with lymphatic invasion, metastasis, and prognosis. FLNC knockdown in esophageal cancer cell lines decreased cell migration in wound healing and transwell migration assays, and invasion in transwell migration assays. Furthermore, FLNC knockdown reduced the amount of activated Rac-1 (GTP-Rac1) and activated Cdc42 (GTP-Cdc42). Our results suggest that FLNC expression is a useful biomarker of ESCC metastatic tendency and that inhibiting FLNC function may be useful to control the metastasis of ESCC.
Volume 8(4)
Pages 6353-6363
Published 2017-1-24
DOI 10.18632/oncotarget.14087
PII 14087
PMID 28031525
PMC PMC5351637
MeSH Adult Aged Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / mortality Carcinoma, Squamous Cell / secondary Cell Line, Tumor Cell Movement* Chi-Square Distribution Esophageal Neoplasms / genetics Esophageal Neoplasms / metabolism* Esophageal Neoplasms / mortality Esophageal Neoplasms / pathology Esophageal Squamous Cell Carcinoma Female Filamins / genetics Filamins / metabolism* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Kaplan-Meier Estimate Lymphatic Metastasis Male Middle Aged Multivariate Analysis Neoplasm Invasiveness Proportional Hazards Models RNA Interference Risk Factors Signal Transduction Time Factors Transfection cdc42 GTP-Binding Protein / genetics cdc42 GTP-Binding Protein / metabolism* rac1 GTP-Binding Protein / genetics rac1 GTP-Binding Protein / metabolism*
IF 5.168
Human and Animal Cells TE-1(RCB1894) TE-8(RCB2098)