| RRC ID |
65940
|
| Author |
Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Sato H, Torimura M.
|
| Title |
Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis.
|
| Journal |
PLoS One
|
| Abstract |
Although it is not yet possible to replace in vivo animal testing completely, the need for a more efficient method for toxicity testing, such as an in vitro cell-based assay, has been widely acknowledged. Previous studies have focused on mRNAs as biomarkers; however, recent studies have revealed that non-coding RNAs (ncRNAs) are also efficient novel biomarkers for toxicity testing. Here, we used deep sequencing analysis (RNA-seq) to identify novel RNA biomarkers, including ncRNAs, that exhibited a substantial response to general chemical toxicity from nine chemicals, and to benzene toxicity specifically. The nine chemicals are listed in the Japan Pollutant Release and Transfer Register as class I designated chemical substances. We used undifferentiated mouse embryonic stem cells (mESCs) as a simplified cell-based toxicity assay. RNA-seq revealed that many mRNAs and ncRNAs responded substantially to the chemical compounds in mESCs. This finding indicates that ncRNAs can be used as novel RNA biomarkers for chemical safety screening.
|
| Volume |
12(7)
|
| Pages |
e0182032
|
| Published |
2017-1-1
|
| DOI |
10.1371/journal.pone.0182032
|
| PII |
PONE-D-17-12312
|
| PMID |
28750099
|
| PMC |
PMC5531504
|
| MeSH |
Animals
Benzene / toxicity
Biomarkers / metabolism*
Cell Line
Chemical Safety*
Down-Regulation / drug effects
Down-Regulation / genetics
Gene Ontology
High-Throughput Nucleotide Sequencing / methods*
Mice
Mice, Inbred C57BL
Mouse Embryonic Stem Cells / drug effects
Mouse Embryonic Stem Cells / metabolism*
RNA / genetics*
RNA / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Untranslated / genetics
RNA, Untranslated / metabolism
Up-Regulation / drug effects
Up-Regulation / genetics
|
| IF |
2.74
|
| Resource |
| Human and Animal Cells |
H-1(AES0001) |
