RRC ID 65940
Author Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Sato H, Torimura M.
Title Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis.
Journal PLoS One
Abstract Although it is not yet possible to replace in vivo animal testing completely, the need for a more efficient method for toxicity testing, such as an in vitro cell-based assay, has been widely acknowledged. Previous studies have focused on mRNAs as biomarkers; however, recent studies have revealed that non-coding RNAs (ncRNAs) are also efficient novel biomarkers for toxicity testing. Here, we used deep sequencing analysis (RNA-seq) to identify novel RNA biomarkers, including ncRNAs, that exhibited a substantial response to general chemical toxicity from nine chemicals, and to benzene toxicity specifically. The nine chemicals are listed in the Japan Pollutant Release and Transfer Register as class I designated chemical substances. We used undifferentiated mouse embryonic stem cells (mESCs) as a simplified cell-based toxicity assay. RNA-seq revealed that many mRNAs and ncRNAs responded substantially to the chemical compounds in mESCs. This finding indicates that ncRNAs can be used as novel RNA biomarkers for chemical safety screening.
Volume 12(7)
Pages e0182032
Published 2017-1-1
DOI 10.1371/journal.pone.0182032
PII PONE-D-17-12312
PMID 28750099
PMC PMC5531504
MeSH Animals Benzene / toxicity Biomarkers / metabolism* Cell Line Chemical Safety* Down-Regulation / drug effects Down-Regulation / genetics Gene Ontology High-Throughput Nucleotide Sequencing / methods* Mice Mice, Inbred C57BL Mouse Embryonic Stem Cells / drug effects Mouse Embryonic Stem Cells / metabolism* RNA / genetics* RNA / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Untranslated / genetics RNA, Untranslated / metabolism Up-Regulation / drug effects Up-Regulation / genetics
IF 2.74
Human and Animal Cells H-1(AES0001)