RRC ID 65957
Author Homma Y, Toga K, Daimon T, Shinoda T, Togawa T.
Title A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori.
Journal Biochem Biophys Res Commun
Abstract Juvenile hormone (JH) plays important roles in the control of many biological processes in insects, such as development, reproduction, and polyphenism. JH is primarily produced in the corpora allata (CA) by specific JH biosynthetic enzymes under strict temporal regulation. In a previous study, we identified a novel putative JH biosynthetic gene, protein tyrosine phosphatase, mitochondrial 1 (PTPMT1), from silkworm, Bombyx mori, whose expression is nearly exclusive in the CA and is correlated with JH synthetic activities during late larval development. In this study, to reveal the function of PTPMT1 in vivo, we generated PTPMT1 knockout silkworms using TALEN. In the knockout mutants, no signs indicating defects in JH activity were observed. Instead, PTPMT1 knockout silkworms showed embryonic lethality, developmental arrest, and 3rd-instar lethality not only in mutants lacking total enzymatic activity but also in mutants lacking mitochondrial translocation signals. Moreover, in PTPMT1 knockout embryos, the expression of two genes encoded by the mitochondrial genome, CYTB and ND3, was decreased, indicating a mitochondrial disorder. These results suggested that PTPMT1 plays conserved vital role(s) reported in vertebrates in insect mitochondria.
Volume 530(4)
Pages 713-718
Published 2020-10-1
DOI 10.1016/j.bbrc.2020.07.124
PII S0006-291X(20)31525-4
PMID 32773109
MeSH Animals Bombyx / embryology Bombyx / genetics Bombyx / growth & development* Bombyx / metabolism Gene Expression Regulation, Developmental Gene Knockout Techniques Insect Proteins / genetics Insect Proteins / metabolism* Juvenile Hormones / genetics Juvenile Hormones / metabolism Mitochondria / genetics Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism*
IF 2.705