RRC ID 65961
Author Paudel A, Hamamoto H, Panthee S, Matsumoto Y, Sekimizu K.
Title Large-Scale Screening and Identification of Novel Pathogenic Staphylococcus aureus Genes Using a Silkworm Infection Model.
Journal J Infect Dis
Abstract The regulatory network of virulence factors produced by the opportunistic pathogen Staphylococcus aureus is unclear and the functions of many uncharacterized genes in its genome remain to be elucidated. In this study, we screened 380 genes whose function was unassigned, utilizing gene-disrupted transposon mutants of the community-acquired methicillin-resistant S. aureus USA300 for pathogenicity in silkworms. We identified 10 strains with reduced silkworm killing ability. Among them, 8 displayed reduced virulence in a mouse model as evidenced by reduced colony-forming units in organs of infected mice. The role of each gene in pathogenicity was further confirmed by complementation and pathogenicity tests in silkworms, where we found that the phenotype was not restored in 1 strain. Additionally, some of the mutants displayed reduced hemolysis, proteolysis, pigment production, and survival in murine RAW 264.7 monocyte-macrophage cells. These newly identified genes involved in virulence will enhance our understanding of the pathogenicity of S. aureus.
Volume 221(11)
Pages 1795-1804
Published 2020-5-11
DOI 10.1093/infdis/jiaa004
PII 5698236
PMID 31912866
MeSH Animals Bombyx / microbiology Disease Models, Animal Female Genes, Bacterial Mice Mice, Inbred C57BL Mutation Phenotype Staphylococcal Infections / genetics Staphylococcal Infections / microbiology* Staphylococcus aureus / genetics* Staphylococcus aureus / metabolism Staphylococcus aureus / pathogenicity Virulence / genetics Whole Genome Sequencing
IF 5.045
Resource
Silkworms