| RRC ID |
66032
|
| 著者 |
Chiba A, Watanabe-Takano H, Terai K, Fukui H, Miyazaki T, Uemura M, Hashimoto H, Hibi M, Fukuhara S, Mochizuki N.
|
| タイトル |
Osteocrin, a peptide secreted from the heart and other tissues, contributes to cranial osteogenesis and chondrogenesis in zebrafish.
|
| ジャーナル |
Development
|
| Abstract |
The heart is an endocrine organ, as cardiomyocytes (CMs) secrete natriuretic peptide (NP) hormones. Since the discovery of NPs, no other peptide hormones that affect remote organs have been identified from the heart. We identified osteocrin (Ostn) as an osteogenesis/chondrogenesis regulatory hormone secreted from CMs in zebrafish. ostn mutant larvae exhibit impaired membranous and chondral bone formation. The impaired bones were recovered by CM-specific overexpression of OSTN. We analyzed the parasphenoid (ps) as a representative of membranous bones. In the shortened ps of ostn morphants, nuclear Yap1/Wwtr1-dependent transcription was increased, suggesting that Ostn might induce the nuclear export of Yap1/Wwtr1 in osteoblasts. Although OSTN is proposed to bind to NPR3 (clearance receptor for NPs) to enhance the binding of NPs to NPR1 or NPR2, OSTN enhanced C-type NP (CNP)-dependent nuclear export of YAP1/WWTR1 of cultured mouse osteoblasts stimulated with saturable CNP. OSTN might therefore activate unidentified receptors that augment protein kinase G signaling mediated by a CNP-NPR2 signaling axis. These data demonstrate that Ostn secreted from the heart contributes to bone formation as an endocrine hormone.
|
| 巻・号 |
144(2)
|
| ページ |
334-344
|
| 公開日 |
2017-1-15
|
| DOI |
10.1242/dev.143354
|
| PII |
dev.143354
|
| PMID |
27993976
|
| MeSH |
Animal Structures / metabolism
Animals
Animals, Genetically Modified
Cells, Cultured
Chondrogenesis / drug effects
Chondrogenesis / genetics*
Embryo, Nonmammalian
HEK293 Cells
Heart / metabolism
Humans
Mice
Myocytes, Cardiac / metabolism*
Organogenesis / drug effects
Organogenesis / genetics
Osteogenesis / drug effects
Osteogenesis / genetics*
Peptide Hormones / genetics
Peptide Hormones / metabolism
Peptide Hormones / pharmacology
Peptide Hormones / physiology
Skull / drug effects
Skull / embryology*
Transcription Factors / metabolism
Transcription Factors / pharmacology
Transcription Factors / physiology*
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish / metabolism
Zebrafish Proteins / metabolism
Zebrafish Proteins / pharmacology
Zebrafish Proteins / physiology*
|
| IF |
5.611
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(UAS:EGFP) |
