論文 - 詳細
| RRC ID | 6614 |
|---|---|
| 著者 | Hossain M, Rashid M, Bhuiyan MA, Nakamura T, Ozaki M, Nagatomo T. |
| タイトル | Mutagenesis of important amino acid reveals unconventional homologous internalization of beta(1)-adrenergic receptor. |
| ジャーナル | Life Sci |
| Abstract |
AIMS:The study was designed to examine the internalization of Asp104Lys mutant of beta(1)-adrenergic receptor (beta(1)-AR) and compared to other mutant (Asp104Ala) and wild type receptors. Moreover, this study needs to perform the role of GRK2 (betaARK1) and beta-arrestin1 on this internalization of Asp104Lys mutant of beta(1)-AR. MAIN METHODS:Binding affinity, functional potency of agonist and agonist-induced internalization were determined for wild type and both mutants of beta(1)-ARs stably expressed in HEK 293 cells as assessed by [(3)H] CGP12177 radioligand. We have performed GRK2 and beta-arrestin1 expression levels by western blot analysis and also performed internalization of this mutant receptor after over expression and deletion of beta-arrestin1 gene. KEY FINDINGS:In the present study, the binding affinity of (-)-isoproterenol for both mutants were significantly decreased compared to wild type. Though the mutant Asp104Ala showed agonist-induced receptor activation, interestingly this mutant was not internalized. However, the mutant Asp104Lys, which showed uncoupling with G protein, was internalized 31.77+/-3.13% from cell surface. Asp104Lys mutant produced the same level of GRK2 expression in (-)-isoproterenol induced stimulation of wild type receptor and addition of (-)-isoproterenol further increased GRK2 expression in mutant receptors. In addition, overexpression of beta-arrestin1 in mutant Asp104Lys promoted (39.75+/-2.19%) and knockdown of beta-arrestin1 by siRNA decreased (3.55+/-1.75%) internalization compared to Asp104Lys mutant of beta(1)-ARs. SIGNIFICANCE:The present studies suggest that Asp104Lys mutant beta(1)-ARs triggers unconventional homologous internalization induced by G protein independent signals, where GRK2 and beta-arrestin1 play an important role for beta(1)-AR internalization. |
| 巻・号 | 85(7-8) |
| ページ | 339-44 |
| 公開日 | 2009-8-12 |
| DOI | 10.1016/j.lfs.2009.06.015 |
| PII | S0024-3205(09)00286-0 |
| PMID | 19580817 |
| MeSH | Adrenergic beta-Agonists / metabolism Adrenergic beta-Antagonists / metabolism Aspartic Acid / genetics* Binding, Competitive Blotting, Western Cell Line Cell Membrane / metabolism Cyclic AMP / metabolism Humans Isoproterenol / metabolism Ligands Lysine / genetics* Mutagenesis, Site-Directed Mutation* Propranolol / metabolism Radioligand Assay Receptors, Adrenergic, beta-1 / genetics Receptors, Adrenergic, beta-1 / metabolism* Transfection |
| IF | 3.647 |
| 引用数 | 1 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | |