| RRC ID |
6621
|
| 著者 |
Senju S, Haruta M, Matsunaga Y, Fukushima S, Ikeda T, Takahashi K, Okita K, Yamanaka S, Nishimura Y.
|
| タイトル |
Characterization of dendritic cells and macrophages generated by directed differentiation from mouse induced pluripotent stem cells.
|
| ジャーナル |
Stem Cells
|
| Abstract |
Methods have been established to generate dendritic cells (DCs) from mouse and human embryonic stem (ES) cells. We designated them as ES-DCs and mouse models have demonstrated the induction of anti-cancer immunity and prevention of autoimmune disease by in vivo administration of genetically engineered ES-DCs. For the future clinical application of ES-DCs, the histoincompatibility between patients to be treated and available human ES cells and the ethical concerns associated with human ES cells may be serious obstacles. However, recently developed induced pluripotent stem (iPS) cell technology is expected to resolve these issues. This report describes the generation and characterization of DCs derived from mouse iPS cells. The iPS cell-derived DCs (iPS-DCs) possessed the characteristics of DCs including the capacity of T-cell-stimulation, antigen-processing and presentation and cytokine production. DNA microarray analyses revealed the upregulation of genes related to antigen-presenting functions during differentiation into iPS-DCs and similarity in gene expression profile in iPS-DCs and bone marrow cell-derived DCs. Genetically modified iPS-DCs expressing antigenic protein primed T-cells specific to the antigen in vivo and elicited efficient antigen-specific anti-tumor immunity. In addition, macrophages were generated from iPS cells (iPS-MP). iPS-MP were comparable with bone marrow cell-derived macrophages in the cell surface phenotype, functions, and gene expression profiles.
|
| 巻・号 |
27(5)
|
| ページ |
1021-31
|
| 公開日 |
2009-5-1
|
| DOI |
10.1002/stem.33
|
| PMID |
19415766
|
| MeSH |
Animals
Antigen Presentation / immunology
Cell Differentiation*
Cell Line
Cell Shape
Cross-Priming / immunology
Cytokines / biosynthesis
Cytotoxicity, Immunologic
Dendritic Cells / cytology*
Dendritic Cells / immunology
Epitopes / immunology
Gene Expression Profiling
Gene Expression Regulation
Macrophages / cytology*
Macrophages / metabolism
Mice
Mice, Inbred C57BL
Phenotype
Pluripotent Stem Cells / cytology*
Pluripotent Stem Cells / metabolism
Surface Properties
T-Lymphocytes / cytology
T-Lymphocytes / immunology
|
| IF |
6.022
|
| 引用数 |
64
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ONCOLOGY
HEMATOLOGY
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
