RRC ID 66228
著者 Murata M, Nakamura K, Kosaka T, Ota N, Osawa A, Muro R, Fujiyama K, Oshima T, Mori H, Wanner BL, Yamada M.
タイトル Cell Lysis Directed by SulA in Response to DNA Damage in Escherichia coli.
ジャーナル Int J Mol Sci
Abstract The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the sulA gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of sulA leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of sulA leads to up-regulation of numerous genes, including soxS. Deletion of soxS markedly reduced the extent of cell lysis by sulA overexpression and soxS overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and mazEF cell death pathways.
巻・号 22(9)
公開日 2021-4-26
DOI 10.3390/ijms22094535
PII ijms22094535
PMID 33926096
PMC PMC8123628
MeSH Amidohydrolases / metabolism Apoptosis / genetics Bacterial Proteins / metabolism Cell Division / genetics DNA Damage / genetics DNA Damage / physiology* Escherichia coli / genetics Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial / genetics Genes, Bacterial / genetics Serine Endopeptidases / metabolism Trans-Activators / metabolism
IF 4.556
リソース情報
原核生物(大腸菌)