Reference - Detail
| RRC ID | 66277 |
|---|---|
| Author | Jang JH, Maeng S, Jung HY, Kim MK, Subramani G. |
| Title | Nocardioides jejuensis sp. nov., isolated from soil. |
| Journal | Antonie Van Leeuwenhoek |
| Abstract |
A Gram-stain positive, asporogenous, aerobic, white -coloured bacterium, designated 18JY15-6T, was isolated from soil from Jeju Island, Korea. Pairwise analysis of the 16S rRNA gene sequence of strain 18JY15-6T indicated high similarity to Nocardioides phosphati DSM 104026T (97.4%), Marmoricola terrae KACC 17308T (96.7%) and Nocardioides jensenii KCTC 0074BPT (96.6%). Phylogenetic analysis revealed that strain 18JY15-6T formed a distinct lineage within the family Nocardioidaceae and is closely related to members of the genus Nocardioides. Genome sequencing of strain 18JY15-6T revealed 3221 total genes, including 3162 protein coding genes, 59 RNA and 31 pseudogenes. Growth was observed at 18-37 °C (optimal 30 °C) in R2A medium at pH 7.0. The major cellular fatty acids of strain 18JY15-6T were identified as C16:0, C18:1ω9c, C18:0 10-methyl, tuberculostearic and C17:0. The fatty acid profile of strain 18JY15-6T was more dissimilar when compared with M. terrae. The only respiratory quinone present was found to be MK-8(H4). The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The results of phylogenetic, biochemical and chemotaxonomic characterisation allow the differentiation of strain 18JY15-6T from N. phosphati WYH11-7T, M. terrae JOS5-1T and N. jensenii NBRC 14755T which supports the conclusion that this strain represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides jejuensis sp. nov. The type strain of Nocardioides jejuensis is 18JY15-6T (= KCTC 49105T = JCM 33182T). |
| Volume | 113(3) |
| Pages | 339-347 |
| Published | 2020-3-1 |
| DOI | 10.1007/s10482-019-01343-y |
| PII | 10.1007/s10482-019-01343-y |
| PMID | 31637662 |
| MeSH | DNA, Bacterial Genome, Bacterial Genomics / methods Phylogeny Propionibacterium / classification* Propionibacterium / genetics Propionibacterium / isolation & purification* RNA, Ribosomal, 16S Soil Microbiology* Whole Genome Sequencing |
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| The most frequently cited source | Blogs |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM33182 |