Reference - Detail
| RRC ID | 66322 |
|---|---|
| Author | Watanabe Y, Watanabe S, Fukui Y, Nishiwaki H. |
| Title | Functional and structural characterization of a novel L-fucose mutarotase involved in non-phosphorylative pathway of L-fucose metabolism. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Mutarotases catalyze the α-β anomeric conversion of monosaccharide, and play a key role in utilizing sugar as enzymes involved in sugar metabolism have specificity for the α- or β-anomer. In spite of the sequential similarity to l-rhamnose mutarotase protein superfamily (COG3254: RhaM), the ACAV_RS08160 gene in Acidovorax avenae ATCC 19860 (AaFucM) is located in a gene cluster related to non-phosphorylative l-fucose and l-galactose metabolism, and transcriptionally induced by these carbon sources; therefore, the physiological role remains unclear. Here, we report that AaFucM possesses mutarotation activity only toward l-fucose by saturation difference (SD) NMR experiments. Moreover, we determined the crystal structures of AaFucM in the apo form and in the l-fucose-bound form at resolutions of 2.21 and 1.75 Å, respectively. The overall structural folding was clearly similar to the RhaM members, differed from the known l-fucose mutarotase (COG4154: FucU), strongly indicating their convergent evolution. The structure-based mutational analyses suggest that Tyr18 is important for catalytic action, and that Gln87 and Trp99 are involved in the l-fucose-specific recognition. |
| Volume | 528(1) |
| Pages | 21-27 |
| Published | 2020-7-12 |
| DOI | 10.1016/j.bbrc.2020.05.094 |
| PII | S0006-291X(20)31015-9 |
| PMID | 32448506 |
| MeSH | Calorimetry Carbohydrate Epimerases / chemistry* Carbohydrate Epimerases / metabolism* Catalytic Domain Comamonadaceae / enzymology Crystallography, X-Ray DNA Mutational Analysis Evolution, Molecular Fucose / chemistry Fucose / metabolism* Magnetic Resonance Spectroscopy Models, Molecular Phosphorylation Structure-Activity Relationship |
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| Total number of mentions | 1 |
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| Resource | |
| General Microbes | JCM20985 |