| RRC ID |
66360
|
| Author |
Khan I, Debnath SC, Yan C, Chen C, Xu Y, Wang WJ, Yu YC, Zheng DQ, Xu JZ, Wang PM.
|
| Title |
Flavobacterium ajazii sp. nov., Isolated from Seaweed of Gouqi Island, China.
|
| Journal |
Curr Microbiol
|
| Abstract |
A novel bacterium designated SSM4.2T was isolated from seaweed of Gouqi Island, which is the center of the Zhoushan fishing ground in the East China Sea. Strain SSM4.2T was Gram-stain-negative, bright yellow-pigmented, short rod-shaped, non-flagellated, non-spore forming, aerobic and motile by gliding. Growth was observed at 4-37 °C (optimum 25-30 °C), pH 6.0-8.0 (optimum pH 7.0) and 0-2.0% (w/v) NaCl (optimum 0%) concentration. The strain was catalase- and oxidase-positive. Menaquinone-6 (MK-6) was found as the sole respiratory quinone and zeaxanthin as the main carotenoid pigment. The predominant fatty acids (≥ 10%) were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c /C16:1 ω6c). The major polar lipid was phosphatidylethanolamine (PE). The genome size was 5.7 Mbp. The DNA G + C content was 34.1 mol%. 16S rRNA gene sequence revealed that strain SSM4.2T belongs to the genus Flavobacterium and shares high-sequence similarity with F. limi KACC 18851T (98.1%), F. hydrophilum KACC 19591T (97.6%), F. defluvii KCTC 12612T (97.1%), F. cheongpyeongense KACC 19592T (97.0%) and F. fluviatile KCTC 52446T (96.9%). Strain SSM4.2T had 73.2-84.6% average nucleotide identity and 19.1-29.4% digital DNA-DNA hybridization values with its closest type strains. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic features, strain SSM4.2T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ajazii sp. nov. is proposed. The type strain is SSM4.2T (= KCTC 72807T = MCCC 1K04370T).
|
| Volume |
77(10)
|
| Pages |
2925-2932
|
| Published |
2020-10-1
|
| DOI |
10.1007/s00284-020-02112-1
|
| PII |
10.1007/s00284-020-02112-1
|
| PMID |
32661679
|
| MeSH |
China
Fatty Acids / analysis
Flavobacterium* / classification
Flavobacterium* / genetics
Flavobacterium* / isolation & purification
Islands
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seaweed* / microbiology
Species Specificity
Vitamin K 2 / analysis
|
| Resource |
| General Microbes |
JCM20475 |
