RRC ID 6639
Author Izawa T, Yamate J, Franklin RJ, Kuwamura M.
Title Abnormal myelinogenesis both in the white and gray matter of the attractin-deficient mv rat.
Journal Brain Res.
Abstract The myelin vacuolation (mv) rat exhibits hypomyelination and vacuole formation in the myelin throughout the CNS, caused by a null mutation in the attractin gene. Myelin alterations in the spinal cord of mv rats progress during postnatal development and are more prominent in the white matter. In contrast, microglial activation is confined to the gray matter of mv rats. We here investigate the distribution and expression patterns of major CNS myelin proteins in the spinal cord of mv rats during the development of the myelin lesions. Immunohistochemical and Western blot analyses demonstrated a considerable reduction in the expression of major CNS myelin proteins both in the white and gray matter of mv rats, which was consistent with the morphological alterations of myelin sheaths. Real-time PCR analysis revealed a significant decrease in expression of proteolipid protein (PLP) mRNA both in the white and gray matter of mv rats. However, there was no significant difference between control and mv rats in the cell number of PLP mRNA-positive oligodendrocytes either in the white or gray matter, suggesting an impairment of myelin protein production by oligodendrocytes. Our results indicate that myelinogenesis but not oligodendrogenesis is severely altered both in the white and gray matter of mv rats.
Volume 1312
Pages 145-55
Published 2010-2-2
DOI 10.1016/j.brainres.2009.11.027
PII S0006-8993(09)02453-6
PMID 19931230
MeSH Animals Central Nervous System / metabolism Central Nervous System / pathology* Demyelinating Diseases / genetics* Demyelinating Diseases / metabolism Demyelinating Diseases / pathology* Disease Models, Animal Gene Expression Regulation / genetics Membrane Proteins / deficiency* Membrane Proteins / genetics Microglia / metabolism Mutation / genetics Myelin Basic Protein / genetics Myelin Basic Protein / metabolism Myelin Proteins Myelin Proteolipid Protein / genetics Myelin Proteolipid Protein / metabolism Myelin Sheath / pathology* Myelin-Associated Glycoprotein / genetics Myelin-Associated Glycoprotein / metabolism Myelin-Oligodendrocyte Glycoprotein Oligodendroglia / metabolism RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Rats, Transgenic Vacuoles / metabolism Vacuoles / pathology
IF 2.929
Times Cited 7
Rats MV/Opu(strainID=187)