| RRC ID |
66417
|
| 著者 |
Meng J, Jin D, Yang J, Lai XH, Pu J, Zhu W, Huang Y, Liang H, Lu S.
|
| タイトル |
Lactobacillus xujianguonis sp. nov., isolated from faeces of Marmota himalayana.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two novel strains (HT111-2T and HT170-2) of the genus Lactobacillus were isolated from Marmota himalayana faecal samples collected on the Qinghai-Tibet Plateau, PR China. The isolates were Gram-stain-positive, rod-shaped, non-spore-forming bacteria with irregular circular colonies. Phylogenetic analysis and comparison of the 16S rRNA gene sequences demonstrated that the two strains form a subcluster and are closest to Lactobacillus hamsteri JCM 6256T (97.3 %) and Lactobacillus amylolyticus DSM 11664T (97.2 %). Phylogenetic analysis of two housekeeping genes (rpoA and pheS) found that strains HT111-2T and HT170-2 had the same closest relatives as the 16S rRNA gene sequence analysis did. The G+C content of strains HT111-2T and HT170-2 were 38.8 mol%. The values of in silico DNA-DNA hybridization with known Lactobacillus species were lower than the threshold (70%). Average nucleotide identity values of strain HT111-2T with L. hamsteri JCM 6256T and L. amylolyticus DSM 11664T were 77.84 % and 76.85 %, respectively. The major fatty acids of strains HT111-2T and HT170-2 were C16 : 0, C18 : 1ω9c and C18 : 0. Results of phenotypic, chemotaxonomic and phylogenetic analyses suggest strains HT111-2T and HT170-2 represent a novel species of the genus Lactobacillus, for which the name Lactobacillus xujianguonis sp. nov. is proposed with HT111-2T (=CGMCC 1.13855T=KCTC 15803T) as the type strain.
|
| 巻・号 |
70(1)
|
| ページ |
11-15
|
| 公開日 |
2020-1-1
|
| DOI |
10.1099/ijsem.0.003598
|
| PMID |
31560297
|
| MeSH |
Animals
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology
Genes, Bacterial
Lactobacillus / classification*
Lactobacillus / isolation & purification
Marmota / microbiology*
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Tibet
|
| リソース情報 |
| 一般微生物 |
JCM6256 |
