| RRC ID |
66418
|
| Author |
Zhu W, Yang J, Lu S, Lai XH, Jin D, Wang X, Pu J, Ren Z, Huang Y, Wu X, Zhang X, Xu J, Xu J.
|
| Title |
Actinomyces qiguomingii sp. nov., isolated from the Pantholops hodgsonii.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China. The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7. The end products from glucose fermentation included ethanol and lactic acid. Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %). The genomic G+C content of strain 410T was 67.4 mol%. Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %. The respiratory quinones were MK-10 (68 %) and MK-9 (32 %). The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1ω9c. The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside. The whole-cell sugars contained rhamnose, ribose and glucose. The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine. The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp. nov. is proposed. The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
|
| Volume |
70(1)
|
| Pages |
58-64
|
| Published |
2020-1-1
|
| DOI |
10.1099/ijsem.0.003709
|
| PMID |
31613742
|
| MeSH |
Actinomyces / classification*
Actinomyces / isolation & purification
Animals
Antelopes / microbiology*
Bacterial Typing Techniques
Base Composition
Cell Wall / chemistry
China
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Tibet
Vitamin K 2 / chemistry
|
| Resource |
| General Microbes |
JCM17565 |
