RRC ID 66421
著者 Zhao J, Yu B, Han C, Cao P, Yu Z, Ju H, Xiang W, Wang X.
タイトル Microbispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.).
ジャーナル Int J Syst Evol Microbiol
Abstract A novel actinomycete, designated strain NEAU-HEGS1-5T, was isolated from the ear of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus Microbispora. The results of 16S rRNA gene sequence analysis showed that the isolate was most closely related to Microbispora bryophytorum NEAU-TX2-2T (99.3 %), Microbispora camponoti 2C-HV3T (99.2 %), Microbispora amethystogenes JCM 3021T (99.1 %) and Microbispora rosea subsp. rosea JCM 3006T (98.5 %). However, two tree-making algorithms supported the position that strain NEAU-HEGS1-5T formed a distinct clade with M. bryophytorum NEAU-TX2-2T, M. camponoti 2C-HV3T and M. rosea subsp. rosea JCM 3006T. Furthermore, multilocus sequence analysis based on the 16S-gyrB-rpoB genes and a combination of DNA-DNA hybridization results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-HEGS1-5T should be classified as representative of a novel species of the genus Microbispora, for which the name Microbispora fusca sp. nov. is proposed. The type strain is NEAU-HEGS1-5T (=CCTCC AA 2019030T=DSM 104648T).
巻・号 70(1)
ページ 139-145
公開日 2020-1-1
DOI 10.1099/ijsem.0.003725
PMID 31622227
MeSH Actinomycetales / classification* Actinomycetales / isolation & purification Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Genes, Bacterial Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Triticum / microbiology*
リソース情報
一般微生物 JCM3021 JCM3006