| RRC ID |
66434
|
| Author |
Zhang D, Gui J, Zheng S, Zhu X, Wu S, Tian Y, Lai Q, Xu H.
|
| Title |
Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Strain GS-14T was isolated from a mangrove sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China. Cells were Gram-stain-negative, strictly aerobic and rod-shaped with a polar flagellum. Optimal growth occurred in the presence of 3-6 % (w/v) NaCl, at pH 6-8 and at a temperature of 37 °C. The predominant polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The major fatty acids (>10 % of the total fatty acids) were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The DNA G+C content was 47.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GS-14T had the highest sequence similarity to Aestuariibacter aggregatus WH169T (96.63 %), Aliiglaciecola coringensis AK49T (96.56 %) and Alteromonas lipolytica JW12T (96.22 %). In addition, the OrthoANIu value and dDDH values calculated from the genomes of strain GS-14T and A. aggregatus WH169T were 79.5 and 21.9 %, respectively. Based on the polyphasic taxonomic results, strain GS-14T is considered to represent a novel species in a new genus, for which the name Marisediminitalea mangrovi gen. nov., sp. nov. is proposed. The type strain of Marisediminitalea mangrovi is GS-14T (=KCTC 72401T=MCCC 1K03622T). Because Aestuariibacter aggregatus WH169T clustered with strain GS-14T in the phylogenetic trees and was clearly separated from the two species within the genus Aestuariibacter, it is reclassified as a member of the genus Marisediminitalea as Marisediminitalea aggregata comb. nov. (type strain WH169T=CGMCC 1.8995T=LMG 25283T). The type species of the genus Marisediminitalea is Marisediminitalea aggregata gen. nov., comb. nov.
|
| Volume |
70(1)
|
| Pages |
457-464
|
| Published |
2020-1-1
|
| DOI |
10.1099/ijsem.0.003773
|
| PMID |
31626587
|
| MeSH |
Alteromonadaceae / classification*
Alteromonadaceae / isolation & purification
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Estuaries*
Fatty Acids / chemistry
Geologic Sediments / microbiology*
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Rhizophoraceae
Sequence Analysis, DNA
Ubiquinone / chemistry
|
| Resource |
| General Microbes |
JCM19197 |
