| RRC ID |
66445
|
| 著者 |
Sakamoto M, Ikeyama N, Toyoda A, Murakami T, Mori H, Iino T, Ohkuma M.
|
| タイトル |
Dialister hominis sp. nov., isolated from human faeces.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
An obligately anaerobic, Gram-stain-negative, rod or coccobacilli organism was isolated from a faecal sample of a healthy Japanese woman. In the 16S rRNA gene sequence analysis, strain 5BBH33T showed the highest 16S rRNA gene sequence similarity to Dialister succinatiphilus YIT 11850T (95.9 %), Dialister propionicifaciens ADV 1053.03T (94.3 %), Dialister micraerophilus DSM 19965T (93.1 %), Dialister invisus DSM 15470T (92.5 %) and Dialister pneumosintes ATCC 33048T (91.4 %). The hsp60 gene sequence analysis also revealed strain 5BBH33T had relatively low hsp60 gene sequence similarities (74.4-85.3 %) to other Dialister species. Strain 5BBH33T showed 21.8-23.9 % in silico DNA-DNA hybridization values with other Dialister species. In addition, the average nucleotide identity values between strain 5BBH33T and other Dialister species ranged from 68.7-74.2 %, indicating that this strain should be considered as new species based on whole-genome relatedness. Strain 5BBH33T was asaccharolytic and largely unreactive for commercial kit. However, its growth was enhanced by adding 1 % (w/v) succinate to the medium; strain 5BBH33T was able to decarboxylate succinate to propionate. The strain 5BBH33T genome contained the enzymes involved in succinate utilization. These results improve our understanding of succinate-utilizing bacteria. On the basis of the collected data, strain 5BBH33T represents a novel species in the genus Dialister, for which the name Dialister hominis sp. nov. is proposed. The type strain of D. hominis is 5BBH33T (=JCM 33369T=DSM 109768T).
|
| 巻・号 |
70(1)
|
| ページ |
589-595
|
| 公開日 |
2020-1-1
|
| DOI |
10.1099/ijsem.0.003797
|
| PMID |
31661048
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Feces / microbiology*
Female
Humans
Japan
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Veillonellaceae / classification*
Veillonellaceae / isolation & purification
|
| リソース情報 |
| 一般微生物 |
JCM33369
JCM10004
JCM15077
JCM17566
JCM17567
JCM17568 |
