| RRC ID |
66477
|
| Author |
Iino T, Kawai S, Yuki M, Dekio I, Ohkuma M, Haruta S.
|
| Title |
Thermaurantimonas aggregans gen. nov., sp. nov., a moderately thermophilic heterotrophic aggregating bacterium isolated from microbial mats at a terrestrial hot spring.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A moderately thermophilic and heterotrophic bacterial strain, LAT, was isolated from microbial mats sampled at a hot spring in Nakabusa, Nagano, Japan. The cells of strain LAT were aerobic, Gram-stain-negative, non-sporulating, non-motile and rod-shaped (2.0-4.1 µm long). The formation of dense cell aggregates in liquid medium was a unique characteristic of the strain. Strain LAT grew optimally at 50°C and at pH 7.5. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbour of strain LAT was Schleiferia thermophila JCM 30197T with 94.1 % sequence similarity. The draft genome sequence of strain LAT (2 671 880 bp) showed 18.0 % digital DNA-DNA hybridization, 70.9 % average nucleotide identity and 72.1 % average amino acid identity (AAI) values in comparison with the genome sequence of S. thermophila JCM 30197T (2 606 763 bp); the 16S rRNA gene sequence similarity and AAI values are lower than the cutoffs used for assignment to a separate genus. On the basis of phenotypic features, major cellular fatty acid composition, genome sequencing and phylogenetic position, a novel genus and species are proposed for strain LAT, to be named Thermaurantimonas aggregans (= JCM 31826T=DSM 106522T).
|
| Volume |
70(2)
|
| Pages |
1117-1121
|
| Published |
2020-2-1
|
| DOI |
10.1099/ijsem.0.003888
|
| PMID |
31778354
|
| MeSH |
Bacterial Typing Techniques
Bacteroidetes / classification*
Bacteroidetes / isolation & purification
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Hot Springs / microbiology*
Japan
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM31826
JCM30197 |
