| RRC ID |
66565
|
| 著者 |
Xie J, Cheng K, Zhao D, Yang G, Qiao Z, Qiu S, Yu X, Liu H, Li T, Feng H, Yao S, Zhai L.
|
| タイトル |
Bacillus aquiflavi sp. nov., isolated from yellow water of strongly flavored Chinese baijiu.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China). Oval endospores were formed at the subtermini of cells with swollen sporangia. The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl). Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid. The main polar lipids of 3 H-10T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid. Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %). The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively. The genomic DNA G+C content was 35.4 mol%. On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp. nov. is proposed. The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
|
| 巻・号 |
70(5)
|
| ページ |
3406-3412
|
| 公開日 |
2020-5-1
|
| DOI |
10.1099/ijsem.0.004185
|
| PMID |
32375947
|
| MeSH |
Alcoholic Beverages / microbiology*
Bacillus / classification*
Bacillus / isolation & purification
Bacterial Typing Techniques
Base Composition
Cell Wall / chemistry
China
DNA, Bacterial / genetics
Diaminopimelic Acid / chemistry
Fatty Acids / chemistry
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Water
|
| リソース情報 |
| 一般微生物 |
JCM33703
JCM33700
JCM33701
JCM33702 |
