| RRC ID |
66571
|
| Author |
Dong K, Lu S, Yang J, Pu J, Lai XH, Jin D, Li J, Zhang G, Wang X, Liang J, Tian Z, Zhang S, Huang Y, Ge Y, Zhou J, Ren Z, Wu X, Huang Y, Wang S, Xu J.
|
| Title |
Nocardioides jishulii sp. nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata).
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides. ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C18 : 1ω9c, iso-C16 : 0 and C17 : 1ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp. nov. is proposed. The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
|
| Volume |
70(6)
|
| Pages |
3665-3672
|
| Published |
2020-6-1
|
| DOI |
10.1099/ijsem.0.004218
|
| PMID |
32416735
|
| MeSH |
Actinobacteria / classification*
Actinobacteria / isolation & purification
Animals
Antelopes / microbiology*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Peptidoglycan / chemistry
Phospholipids / chemistry
Phylogeny*
Pigmentation
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Tibet
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
| Resource |
| General Microbes |
JCM33496 |
