RRC ID 6659
著者 Cornillon S, Pech E, Benghezal M, Ravanel K, Gaynor E, Letourneur F, Brückert F, Cosson P.
タイトル Phg1p is a nine-transmembrane protein superfamily member involved in dictyostelium adhesion and phagocytosis.
ジャーナル J. Biol. Chem.
Abstract To identify the molecular mechanisms involved in phagocytosis, we generated random insertion mutants of Dictyostelium discoideum and selected two mutants defective for phagocytosis. Both represented insertions in the same gene, named PHG1. This gene encodes a polytopic membrane protein with an N-terminal lumenal domain and nine potential transmembrane segments. Homologous genes can be identified in many species; however, their function is yet to be elucidated. Disruption of PHG1 caused a selective defect in phagocytosis of latex beads and Escherichia coli, but not Klebsiella aerogenes bacteria. This defect in phagocytosis was caused by a decrease in the adhesion of mutant cells to phagocytosed particles. These results indicate that the Phg1 protein is involved in the adhesion of Dictyostelium to various substrates, a crucial event of phagocytosis and demonstrate the usefulness of a genetic approach to dissect the molecular events involved in the phagocytic process.
巻・号 275(44)
ページ 34287-92
公開日 2000-11-3
DOI 10.1074/jbc.M006725200
PII M006725200
PMID 10944536
MeSH Amino Acid Sequence Animals Cell Adhesion / physiology* DNA, Protozoan Dictyostelium / genetics Dictyostelium / physiology* Dictyostelium / ultrastructure Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / physiology* Microscopy, Electron, Scanning Molecular Sequence Data Mutation Phagocytosis / physiology* Phenotype Sequence Homology, Amino Acid
IF 4.125
引用数 130
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
細胞性粘菌 G22483