| RRC ID |
66600
|
| Author |
Jiang Z, Duan Y, Yang X, Yao B, Zeng T, Wang X, Feng Q, Qi M, Yang Q, Zhang X.
|
| Title |
Nitratireductor alexandrii sp. nov., from phycosphere microbiota of toxic marine dinoflagellate Alexandrium tamarense.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A taxonomic study was carried out on a novel algae-associated bacterial strain Z3-1T, which was isolated from phycosphere microbiota of toxic marine dinoflagellate Alexandrium tamarense 880. Cells of strain Z3-1T were Gram-stain-negative, rod-shaped and strictly aerobic and were motile by means of flagella. Strain Z3-1T grew at 25-42 °C, pH 5.0-10.0 and 1.0-5.0 % (w/v) NaCl. Strain Z3-1T reduced nitrate to nitrite, but did not reduce nitrite to nitrogen gas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Z3-1T belongs to the genus Nitratireductor showing the highest sequence similarity (97.0 %) to Nitratireductor basaltis JCM 14935T. The average nucleotide identity and digital DNA-DNA hybridization relatedness between strain Z3-1T and type strains of genus Nitratireductor with available genome sequences were in the ranges of 72.4-74.4 % and 22.7-23.3 %, respectively. The major fatty acids were summed in feature 8 (C18:1ω7c and/or C18:1ω6c), C19:0ω8c cyclo, C18:1ω7c 11-metyl and iso-C17:0. The major polar lipids were determined as diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and four unidentified polar lipids. The genomic DNA G+C content calculated from genome sequence was 65.6 mol%. Based on genotypic, chemotaxonomic and phenotypic data obtained, strain Z3-1T represents a novel species of the genus Nitratireductor, for which the name Nitratireductor alexandrii sp. nov. is proposed with the type strain Z3-1T (=KCTC 62458T=CCTCC AB 2017227T).
|
| Volume |
70(7)
|
| Pages |
4390-4397
|
| Published |
2020-7-1
|
| DOI |
10.1099/ijsem.0.004302
|
| PMID |
32589575
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Dinoflagellida / microbiology*
Fatty Acids / chemistry
Microbiota
Nucleic Acid Hybridization
Phospholipids / chemistry
Phyllobacteriaceae / classification*
Phyllobacteriaceae / isolation & purification
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM14935
JCM17288
JCM21793
JCM14851 |
