論文 - 詳細
| RRC ID | 66601 |
|---|---|
| 著者 | Sun C, Zeng X, Lai Q, Wang Z, Shao Z. |
| タイトル | Mangrovibacterium lignilyticum sp. nov., a facultatively anaerobic lignin-degrading bacterium isolated from mangrove sediment. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
An alkali lignin-degrading, Gram-stain-negative, rod-shaped, non-motile and facultatively anaerobic bacterium, designated BM_7T, was isolated from mangrove sediment of the supralittoral zone in the Jiulong river estuary, PR China. The cells of strain BM_7T were 0.4-0.6 µm wide and 1.0-8.5 µm long. Oxidase and catalase activities were positive. Strain BM_7T could grow at 10-37 °C (optimum, 25-28 °C), at pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 0.5-6 % (w/v) NaCl (optimum, 2%). Phylogenetic analysis of 16S rRNA gene sequences indicated that strain BM_7T belonged to the genus Mangrovibacterium of the family Prolixibacteraceae. It showed the highest similarity to Mangrovibacterium diazotrophicum JCM 19152T (96.8 %), followed by Mangrovibacterium marinum KCTC 42253T (96.1%). The values of average nucleotide identity and DNA-DNA hybridization were calculated as 76.9, 24.3 and 76.1, 17.4 % between strain BM_7T with M. diazotrophicum JCM 19152T and M. marinum KCTC 42253T, respectively. The major respiratory quinone of strain BM_7T was MK-7. The polar lipids were detected as phosphatidylethanolamine, three unidentified phospholipids and four unidentified aminolipids. The dominant fatty acids consisted of iso-C15 : 0, anteiso-C15 : 0, C15 : 1 ω6c, iso-C17 : 0 3-OH, C17 : 1 ω6c, C17 : 0 3-OH and C17 : 0. The genome size of strain BM_7T is 5.6 Mb, with G+C content of 43.4 mol%. Based on the phylogenetic and phenotypic characteristics, strain BM_7T was considered to represent a novel species of the genus Mangrovibacterium, and the name Mangrovibacterium lignilyticum sp. nov. is proposed. The type strain is BM_7T (=MCCC 1A15882T=KCTC 72696T). |
| 巻・号 | 70(8) |
| ページ | 4502-4507 |
| 公開日 | 2020-8-1 |
| DOI | 10.1099/ijsem.0.004305 |
| PMID | 32598276 |
| MeSH | Bacterial Typing Techniques Bacteroidetes / classification* Bacteroidetes / isolation & purification Base Composition China DNA, Bacterial / genetics Estuaries* Fatty Acids / chemistry Geologic Sediments / microbiology* Lignin / metabolism* Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM19152 |