| RRC ID |
66610
|
| Author |
Li T, Zhuo Y, Jin CZ, Wu X, Ko SR, Jin FJ, Ahn CY, Oh HM, Lee HG, Jin L.
|
| Title |
Genomic insights into a novel species Rhodoferax aquaticus sp. nov., isolated from freshwater.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel non-phototrophic member of the genus Rhodoferax was obtained from freshwater. The purpose of this study was to analyse the genome of a nonphototrophic strain and propose a new species based on its phylogenetic, genomic, physiological and chemotaxonomic characteristics. The results of phylogenetic analysis based on 16S rRNA gene sequences supports that the strain, designated Gr-4T, has a close relationship to the genus Rhodoferax. The observed average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Gr-4T and its closest related strains were 72.3-74.6 % and 21.9-22.8 %, respectively. These values were much lower than the species separation thresholds for ANI or dDDH of 95-96 and 70 %, respectively, and in fact fall in the intergeneric range. Strain Gr-4T does not contain RuBisCO-related genes, but does contain GS/GOGAT pathway-related genes enabling nitrate ammonification. A polyphasic study and a genomic-level investigation were done to establish the taxonomic status of strain Gr-4T. Based on the phylogenetic, genomic and physiological differences, it is proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax aquaticus sp. nov. with isolate Gr-4T (=KCTC 32394T=JCM 19166T) as the type strain.
|
| Volume |
70(8)
|
| Pages |
4653-4660
|
| Published |
2020-8-1
|
| DOI |
10.1099/ijsem.0.004325
|
| PMID |
32672530
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Comamonadaceae / classification*
Comamonadaceae / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Fresh Water / microbiology*
Genes, Bacterial
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM19166 |
