| RRC ID |
66658
|
| Author |
Enomoto S, Shimane Y, Ihara K, Kamekura M, Itoh T, Ohkuma M, Takahashi-Ando N, Fukushima Y, Yoshida Y, Usami R, Takai K, Minegishi H.
|
| Title |
Haloarcula mannanilytica sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample. Cells were motile, rod-shaped, and stained Gram-negative. Colonies were pink pigmented. Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C). The DNA G+C content was 62.1 mol% (genome). The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T. The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2. The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2. Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp. nov. is proposed. The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
|
| Volume |
70(12)
|
| Pages |
6331-6337
|
| Published |
2020-12-1
|
| DOI |
10.1099/ijsem.0.004535
|
| PMID |
33095133
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Archaeal / genetics
DNA, Bacterial / genetics
Fatty Acids / chemistry
Galactose / analogs & derivatives
Haloarcula / classification*
Haloarcula / isolation & purification
Japan
Mannans / metabolism
Phylogeny*
Pigmentation
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / analysis*
|
| Resource |
| General Microbes |
JCM33835
JCM7785
JCM8911 |
