| RRC ID |
66660
|
| 著者 |
Goh CBS, Wong LW, Parimannan S, Rajandas H, Loke S, Croft L, Yule CM, Pasbakhsh P, Lee SM, Tan JBL.
|
| タイトル |
Chitinophaga extrema sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a Koompassia malaccensis tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39' 04.7' N 101° 17' 43.7'' E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1T belongs to the genus Chitinophaga with the greatest sequence similarity to Chitinophaga terrae KP01T (97.65 %), Chitinophaga jiangningensis DSM27406T (97.58 %), and Chitinophaga dinghuensis DHOC24T (97.17 %). The major fatty acids of strain Mgbs1T (>10 %) are iso-C15 : 0, C16 : 1 ω5c and iso-C17 : 0 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1T has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA-DNA hybridization (DDH) score between strain Mgbs1T and C. jiangningensis DSM27406T was 15.9 %, while in silico DDH values of strain Mgbs1T against C. dinghuensis DHOC24T and C. terrae KP01T were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1T against all three reference strains are 73.2 %. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga extrema sp. nov. is proposed (=DSM 108835T=JCM 33276T).
|
| 巻・号 |
70(12)
|
| ページ |
6355-6363
|
| 公開日 |
2020-12-1
|
| DOI |
10.1099/ijsem.0.004539
|
| PMID |
33146596
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Forests
Gammaproteobacteria / classification*
Gammaproteobacteria / isolation & purification
Malaysia
Nucleic Acid Hybridization
Phylogeny*
Plant Leaves
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
Wetlands*
|
| リソース情報 |
| 一般微生物 |
JCM33276 |
