RRC ID 6675
Author Nomura T, Carlton JM, Baird JK, del Portillo HA, Fryauff DJ, Rathore D, Fidock DA, Su X, Collins WE, McCutchan TF, Wootton JC, Wellems TE.
Title Evidence for different mechanisms of chloroquine resistance in 2 Plasmodium species that cause human malaria.
Journal J Infect Dis
Abstract Chloroquine (CQ)-resistant Plasmodium vivax malaria was first reported 12 years ago, nearly 30 years after the recognition of CQ-resistant P. falciparum. Loss of CQ efficacy now poses a severe problem for the prevention and treatment of both diseases. Mutations in a digestive vacuole protein encoded by a 13-exon gene, pfcrt, were shown recently to have a central role in the CQ resistance (CQR) of P. falciparum. Whether mutations in pfcrt orthologues of other Plasmodium species are involved in CQR remains an open question. This report describes pfcrt homologues from P. vivax, P. knowlesi, P. berghei, and Dictyostelium discoideum. Synteny between the P. falciparum and P. vivax genes is demonstrated. However, a survey of patient isolates and monkey-adapted lines has shown no association between in vivo CQR and codon mutations in the P. vivax gene. This is evidence that the molecular events underlying P. vivax CQR differ from those in P. falciparum.
Volume 183(11)
Pages 1653-61
Published 2001-6-1
DOI 10.1086/320707
PII JID001437
PMID 11343215
MeSH Amino Acid Sequence Animals Chloroquine / pharmacology* Codon Dictyostelium / chemistry Dictyostelium / genetics Drug Resistance Humans Molecular Chaperones / genetics* Molecular Sequence Data Mutation Parasitic Sensitivity Tests Plasmodium / chemistry Plasmodium / drug effects* Plasmodium / genetics Sequence Alignment
IF 5.022
Times Cited 133
Cellular slime molds G03081