RRC ID 66876
Author Yamazaki SI, Hashimura H, Morimoto YV, Miyanaga Y, Matsuoka S, Kamimura Y, Ueda M.
Title Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds.
Journal Biochem Biophys Res Commun
Abstract Collective cell migration is a key process during the development of multicellular organisms, in which the migrations of individual cells are coordinated through chemical guidance and physical contact between cells. Talin has been implicated in mechanical linkage between actin-based motile machinery and adhesion molecules, but how talin contributes to collective cell migration is unclear. Here we show that talin B is involved in chemical coordination between cells for collective cell migration at the multicellular mound stage in the development of Dictyostelium discoideum. From early aggregation to the mound formation, talB-null cells exhibited collective migration normally with cAMP relay. Subsequently, talB-null cells showed developmental arrest at the mound stage, and at the same time, they had impaired collective migration and cAMP relay, while wild-type cells exhibited rotational cell migration continuously in concert with cAMP relay during the mound stage. Genetic suppression of PI3K activity partially restored talB-null phenotypes in collective cell migration and cAMP relay. Overall, our observations suggest that talin B regulates chemical coordination via PI3K-mediated signaling in a stage-specific manner for the multicellular development of Dictyostelium cells.
Volume 525(2)
Pages 372-377
Published 2020-4-30
DOI 10.1016/j.bbrc.2020.02.060
PII S0006-291X(20)30328-4
PMID 32098673
IF 2.705
Resource
Cellular slime molds