RRC ID 66878
Author Kundert P, Sarrion-Perdigones A, Gonzalez Y, Katoh-Kurasawa M, Hirose S, Lehmann P, Venken KJT, Shaulsky G.
Title A GoldenBraid cloning system for synthetic biology in social amoebae.
Journal Nucleic Acids Res
Abstract GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
Volume 48(8)
Pages 4139-4146
Published 2020-5-7
DOI 10.1093/nar/gkaa185
PII 5813799
PMID 32232356
IF 11.147
Resource
Cellular slime molds