論文 - 詳細
| RRC ID | 66878 |
|---|---|
| 著者 | Kundert P, Sarrion-Perdigones A, Gonzalez Y, Katoh-Kurasawa M, Hirose S, Lehmann P, Venken KJT, Shaulsky G. |
| タイトル | A GoldenBraid cloning system for synthetic biology in social amoebae. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid. |
| 巻・号 | 48(8) |
| ページ | 4139-4146 |
| 公開日 | 2020-5-7 |
| DOI | 10.1093/nar/gkaa185 |
| PII | 5813799 |
| PMID | 32232356 |
| PMC | PMC7192589 |
| MeSH | Chemotaxis Cloning, Molecular / methods* Cyclic AMP / physiology Dictyostelium / genetics* Dictyostelium / physiology Luminescent Proteins / genetics Synthetic Biology / methods |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | AX4 |