| RRC ID |
66878
|
| 著者 |
Kundert P, Sarrion-Perdigones A, Gonzalez Y, Katoh-Kurasawa M, Hirose S, Lehmann P, Venken KJT, Shaulsky G.
|
| タイトル |
A GoldenBraid cloning system for synthetic biology in social amoebae.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
|
| 巻・号 |
48(8)
|
| ページ |
4139-4146
|
| 公開日 |
2020-5-7
|
| DOI |
10.1093/nar/gkaa185
|
| PII |
5813799
|
| PMID |
32232356
|
| PMC |
PMC7192589
|
| MeSH |
Chemotaxis
Cloning, Molecular / methods*
Cyclic AMP / physiology
Dictyostelium / genetics*
Dictyostelium / physiology
Luminescent Proteins / genetics
Synthetic Biology / methods
|
| IF |
11.502
|
| リソース情報 |
| 細胞性粘菌 |
AX4 |
