| RRC ID |
66880
|
| 著者 |
Itoh A, Nakazato S, Wakabayashi H, Hamano A, Shenton MR, Miyamoto K, Mitsuhashi W, Okada K, Toyomasu T.
|
| タイトル |
Functional kaurene-synthase-like diterpene synthases lacking a gamma domain are widely present in Oryza and related species.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Various diterpene synthases have been functionally identified in cultivated rice (Oryza sativa). These are the homologs of ent-copalyl diphosphate (ent-CDP) synthase and ent-kaurene synthase (KS) that are responsible for the biosynthesis of gibberellins, diterpenoid phytohormones. We isolated a cDNA encoding full-length OsKSL12, a previously uncharacterized KS like (KSL) enzyme that consists of a β-domain and an α-domain with an active center, but lacks an N-terminal γ-domain. Functional analysis using a bacterial expression system showed that recombinant OsKSL12 converted ent-CDP into ent-manool or ent-13-epi-manool. Comparative genomics revealed that functional OsKSL12 homologs exist in diverse wild species in the Oryzeae- Oryza nivara (Oryza rufipogon), Oryza coarctata, Oryza granulata, Leersia perrieri and Leersia tisseranti. KSL12 homologs in O. granulata, L. perrieri and L. tisseranti preferentially reacted with GGDP rather than ent-CDP, resulting in geranyllinalool rather than ent-manool or ent-13-epi-manool as the main product, meaning that KSL12 functionally diversified during evolution in the Oryzeae.
|
| 巻・号 |
85(9)
|
| ページ |
1945-1952
|
| 公開日 |
2021-8-25
|
| DOI |
10.1093/bbb/zbab127
|
| PII |
6318364
|
| PMID |
34244709
|
| MeSH |
Alkyl and Aryl Transferases / analysis*
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
Diterpenes / chemistry*
Evolution, Molecular
Genome, Plant
Oryza / enzymology*
Oryza / genetics
Phylogeny
Protein Domains
|
| IF |
1.516
|
| リソース情報 |
| イネ |
W0106
W0002
W0003
W1167
W0008
W0001
W0551
W1529
W1345
W0510 |
