RRC ID 66882
Author Tanvir MIO, Itoh G, Adachi H, Yumura S.
Title Dynamics of Myosin II Filaments during Wound Repair in Dividing Cells.
Journal Cells
Abstract Wound repair of cell membranes is essential for cell survival. Myosin II contributes to wound pore closure by interacting with actin filaments in larger cells; however, its role in smaller cells is unclear. In this study, we observed wound repair in dividing cells for the first time. The cell membrane in the cleavage furrow, where myosin II localized, was wounded by laserporation. Upon wounding, actin transiently accumulated, and myosin II transiently disappeared from the wound site. Ca2+ influx from the external medium triggered both actin and myosin II dynamics. Inhibition of calmodulin reduced both actin and myosin II dynamics. The wound closure time in myosin II-null cells was the same as that in wild-type cells, suggesting that myosin II is not essential for wound repair. We also found that disassembly of myosin II filaments by phosphorylation did not contribute to their disappearance, indicating a novel mechanism for myosin II delocalization from the cortex. Furthermore, we observed that several furrow-localizing proteins such as GAPA, PakA, myosin heavy chain kinase C, PTEN, and dynamin disappeared upon wounding. Herein, we discuss the possible mechanisms of myosin dynamics during wound repair.
Volume 10(5)
Published 2021-5-17
DOI 10.3390/cells10051229
PII cells10051229
PMID 34067877
PMC PMC8156316
MeSH Calcium / metabolism Calcium Signaling Cell Division* Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / metabolism* Kinetics Microscopy, Fluorescence Microscopy, Video Mutation Myosin Type II / genetics Myosin Type II / metabolism* Protozoan Proteins / genetics Protozoan Proteins / metabolism* Time-Lapse Imaging Wound Healing*
IF 4.366
Cellular slime molds GFP-myosin II heavy chain GFP-3Ala myosin II GFP-E476K myosin II GFP-lifeact Dd-GCaMP6s GFP-GAPA