論文 - 詳細
RRC ID | 6702 |
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著者 | Matsuoka S, Kuwayama H, Ikeno D, Oyama M, Maeda M. |
タイトル | Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium. |
ジャーナル | Dev Growth Differ |
Abstract |
We have previously reported that cells of Dictyostelium discoideum lacking the fatty acid oxidation enzyme MFE1 accumulate excess cyclopropane fatty acids from ingested bacteria. Cells in which mfeA(-) is disrupted fail to develop when grown in association with bacteria but form normal fruiting bodies when grown in axenic media. Bacterially grown mfeA(-) cells express the genes for the cyclic AMP (cAMP) receptor (carA) and adenylyl cyclase (acaA) but fail to respond to a cAMP pulse by synthesis of additional cAMP which normally relays the signal. Moreover, they do not accumulate the adhesion protein, gp80, which is encoded by the cAMP-induced gene, csaA. As a consequence, they do not acquire developmentally regulated EDTA-resistant cell-cell adhesion. When mutant cells are mixed with wild-type cells and allowed to develop together, they co-aggregate and differentiate into both spores and stalk cells. Thus, most of the developmental consequences of excess cyclopropane fatty acids appear to result from impaired cAMP relay. |
巻・号 | 46(2) |
ページ | 195-9 |
公開日 | 2004-4-1 |
DOI | 10.1111/j.1440-169X.2004.00732.x |
PII | DGD732 |
PMID | 15066197 |
MeSH | Adenylyl Cyclases / genetics Adenylyl Cyclases / metabolism* Animals Cell Adhesion / genetics Cell Adhesion / physiology Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Cell Differentiation / genetics Cell Differentiation / physiology Cyclic AMP / metabolism* Cyclopropanes / metabolism Dictyostelium / genetics Dictyostelium / physiology* Fatty Acids / metabolism Gene Expression Regulation / genetics Gene Expression Regulation / physiology Genes, Protozoan / genetics Morphogenesis / genetics Morphogenesis / physiology Multienzyme Complexes / deficiency Multienzyme Complexes / genetics Multienzyme Complexes / metabolism* Peroxisomes / genetics Peroxisomes / metabolism* Protozoan Proteins / genetics Protozoan Proteins / metabolism* Receptors, Cyclic AMP / metabolism* Signal Transduction / genetics Signal Transduction / physiology |
IF | 1.723 |
引用数 | 2 |
WOS 分野 | CELL BIOLOGY DEVELOPMENTAL BIOLOGY |
リソース情報 | |
細胞性粘菌 | G23680 G01435 G21980 S90282 |