RRC ID 6702
著者 Matsuoka S, Kuwayama H, Ikeno D, Oyama M, Maeda M.
タイトル Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium.
ジャーナル Dev Growth Differ
Abstract We have previously reported that cells of Dictyostelium discoideum lacking the fatty acid oxidation enzyme MFE1 accumulate excess cyclopropane fatty acids from ingested bacteria. Cells in which mfeA(-) is disrupted fail to develop when grown in association with bacteria but form normal fruiting bodies when grown in axenic media. Bacterially grown mfeA(-) cells express the genes for the cyclic AMP (cAMP) receptor (carA) and adenylyl cyclase (acaA) but fail to respond to a cAMP pulse by synthesis of additional cAMP which normally relays the signal. Moreover, they do not accumulate the adhesion protein, gp80, which is encoded by the cAMP-induced gene, csaA. As a consequence, they do not acquire developmentally regulated EDTA-resistant cell-cell adhesion. When mutant cells are mixed with wild-type cells and allowed to develop together, they co-aggregate and differentiate into both spores and stalk cells. Thus, most of the developmental consequences of excess cyclopropane fatty acids appear to result from impaired cAMP relay.
巻・号 46(2)
ページ 195-9
公開日 2004-4-1
DOI 10.1111/j.1440-169X.2004.00732.x
PII DGD732
PMID 15066197
MeSH Adenylyl Cyclases / genetics Adenylyl Cyclases / metabolism* Animals Cell Adhesion / genetics Cell Adhesion / physiology Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Cell Differentiation / genetics Cell Differentiation / physiology Cyclic AMP / metabolism* Cyclopropanes / metabolism Dictyostelium / genetics Dictyostelium / physiology* Fatty Acids / metabolism Gene Expression Regulation / genetics Gene Expression Regulation / physiology Genes, Protozoan / genetics Morphogenesis / genetics Morphogenesis / physiology Multienzyme Complexes / deficiency Multienzyme Complexes / genetics Multienzyme Complexes / metabolism* Peroxisomes / genetics Peroxisomes / metabolism* Protozoan Proteins / genetics Protozoan Proteins / metabolism* Receptors, Cyclic AMP / metabolism* Signal Transduction / genetics Signal Transduction / physiology
IF 1.723
引用数 2
WOS 分野 CELL BIOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 G23680 G01435 G21980 S90282