RRC ID |
6709
|
Author |
Morita T, Amagai A, Maeda Y.
|
Title |
Translocation of the Dictyostelium TRAP1 homologue to mitochondria induces a novel prestarvation response.
|
Journal |
J Cell Sci
|
Abstract |
Dd-TRAP1 is a Dictyostelium homologue of tumor necrosis factor receptor-associated protein 1 (TRAP-1). Dd-TRAP1 is located in the cortex of cells growing at a low density, but was found to be translocated to mitochondria with the help of a novel prestarvation factor that was accumulated in growth medium along with increased cell densities. The knockdown mutant of Dd-TRAP1 (TRAP1-RNAi cells) exhibited a significant defect in prestarvation response. Although TRAP1-RNAi cells showed normal expressions of classical prestarvation genes [dscA (discoidin I) and car1 (carA; cAMP receptor)], the expression of differentiation-associated genes (dia1 and dia3) induced by the prestarvation response were markedly repressed. By contrast, transformants overexpressing Dd-TRAP1 showed an early prestarvation response and also increased expression of dia1 and dia3 in a cell-density-dependent manner. Importantly, introduction of Dd-TRAP1 antibody into D. discoideum Ax-2 cells by electroporation inhibited the translocation of Dd-TRAP1 from the cortex to mitochondria and greatly inhibited the initiation of differentiation. Taken together, these results indicate that Dd-TRAP1 is translocated to mitochondria by sensing the cell density in growth medium and enhances the early developmental program through a novel prestarvation response.
|
Volume |
117(Pt 24)
|
Pages |
5759-70
|
Published |
2004-11-15
|
DOI |
10.1242/jcs.01499
|
PII |
jcs.01499
|
PMID |
15507488
|
MeSH |
Animals
Blotting, Northern
Cell Differentiation
Cyclic AMP / metabolism
Cytoplasmic Granules / metabolism
Dictyostelium
Discoidins
Electroporation
HSP90 Heat-Shock Proteins / metabolism
Lectins / metabolism
Microscopy, Fluorescence
Mitochondria / metabolism*
Models, Genetic
Mutation
Protein Transport
Protozoan Proteins / genetics
Protozoan Proteins / metabolism*
RNA / metabolism
RNA Interference
Receptors, Cyclic AMP / metabolism
Spores, Protozoan / growth & development
Time Factors
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / metabolism*
|
IF |
4.573
|
Times Cited |
22
|
WOS Category
|
CELL BIOLOGY
|
Resource |
Cellular slime molds |
G01070
S90287
S90288 |