RRC ID 6710
著者 Singh SR, Rekha N, Pillai B, Singh V, Naorem A, Sampath V, Srinivasan N, Sadhale PP.
タイトル Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.
ジャーナル Nucleic Acids Res
Abstract The subcomplex of Rpb4 and Rpb7 subunits of RNA pol II in Saccharomyces cerevisiae is known to be an important determinant of transcription under a variety of physiological stresses. In S.cerevisiae, RPB7 is essential for cell viability while rpb4 null strains are temperature sensitive at low and high temperatures. The rpb4 null strain also shows defect in sporulation and a predisposed state of pseudohyphal growth. We show here that, apart from S.cerevisiae Rpb7, the Rpb7 homologs from other lower eukaryotes like Schizosaccharomyces pombe, Candida albicans and Dictyostelium discoideum can complement for the absence of S.cerevisiae RPB7. This is the first report where we have shown that both the C.albicans and D.discoideum homologs are functional orthologs of the yeast RPB7. We also show that high expression levels of S.cerevisiae RPB7 and its homologs rescue the sporulation defect of rpb4 homozygous null diploids, but only some of them cause significant enhancement of the pseudohyphal phenotype. Structural modeling of Rpb7 and its homologs show a high degree of conservation in the overall structure. This study indicates a structural and functional conservation of different Rpb7 across species and also a conserved role of Rpb7 in the subcomplex with respect to nutritional stress.
巻・号 32(1)
ページ 201-10
公開日 2004-1-1
DOI 10.1093/nar/gkh163
PII 32/1/201
PMID 14704357
PMC PMC373273
MeSH Amino Acid Sequence Animals Candida albicans / enzymology* Candida albicans / genetics Conserved Sequence* Dictyostelium / enzymology* Dictyostelium / genetics Evolution, Molecular* Gene Deletion Genetic Complementation Test Methanococcus / enzymology Models, Molecular Molecular Sequence Data Phenotype Protein Structure, Tertiary RNA Polymerase II / chemistry RNA Polymerase II / genetics RNA Polymerase II / metabolism* Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Sequence Alignment
IF 11.502
引用数 9
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
細胞性粘菌 G00906