論文 - 詳細
RRC ID | 67113 |
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著者 | Jolly SM, Gainetdinov I, Jouravleva K, Zhang H, Strittmatter L, Bailey SM, Hendricks GM, Dhabaria A, Ueberheide B, Zamore PD. |
タイトル | Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. |
ジャーナル | Cell |
Abstract |
In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15- to 18-nt DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of gyrase and TtAgo activity slows growth and produces long sausage-like filaments in which the individual bacteria are linked by DNA. Finally, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication. |
巻・号 | 182(6) |
ページ | 1545-1559.e18 |
公開日 | 2020-9-17 |
DOI | 10.1016/j.cell.2020.07.036 |
PII | S0092-8674(20)30944-2 |
PMID | 32846159 |
PMC | PMC7502556 |
MeSH | Argonaute Proteins / genetics Argonaute Proteins / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cell Survival / drug effects Cell Survival / genetics Chromosomes / metabolism Ciprofloxacin / pharmacology DNA / genetics DNA / metabolism* DNA Gyrase / metabolism* DNA Replication / drug effects DNA Replication / genetics* Endonucleases / metabolism Microscopy, Electron, Scanning Microscopy, Electron, Transmission Models, Molecular Recombinant Proteins Recombination, Genetic / drug effects Recombination, Genetic / genetics Single Molecule Imaging Tandem Mass Spectrometry Thermus thermophilus / genetics Thermus thermophilus / growth & development Thermus thermophilus / metabolism* Thermus thermophilus / ultrastructure Topoisomerase II Inhibitors / pharmacology |
IF | 38.637 |
リソース情報 | |
遺伝子材料 | pUC18-HTK (with promoter) (RDB03436) |