RRC ID 6714
Author Mayanagi T, Amagai A, Maeda Y.
Title DNG1, a Dictyostelium homologue of tumor suppressor ING1 regulates differentiation of Dictyostelium cells.
Journal Cell Mol Life Sci
Abstract dng1 is a Dictyostelium homologue of the mammalian tumor suppressor ING gene. DNG1 protein localizes in the nucleus, and has a highly conserved PHD finger domain found in chromatin-remodeling proteins. Both dng1 disruption and overexpression impaired cell proliferation. In dng1-null cells, the progression of differentiation was delayed in a cell-density-dependent manner, and many tiny aggregates were formed. Exogenously applied cAMP pulses reversed the inhibitory effect caused by dng1 disruption on the aggregation during early development, but formation of tiny aggregates was not restored. dng1-overexpressing cells acquired the ability to undergo chemotaxis to cAMP earlier and exhibited enhanced differentiation. These phenotypes were found to be coupled with altered expressions of early genes such as cAMP receptor 1 (car1) and contact site A (csA). Furthermore, disordered histone modifications were demonstrated in dng1-null cells. These results suggest a regulatory role of dng1 in the transition of cells from growth to differentiation.
Volume 62(15)
Pages 1734-43
Published 2005-8
DOI 10.1007/s00018-005-4570-0
PMID 16003496
MeSH Amino Acid Sequence Animals Caffeine / pharmacology Cell Cycle Proteins / chemistry Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Cyclic AMP / pharmacology DNA-Binding Proteins / chemistry Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / growth & development* Gene Expression Regulation, Developmental Genes, Tumor Suppressor Histones / metabolism Molecular Sequence Data Mutation Transcription Factors / analysis Transcription Factors / genetics Transcription Factors / physiology*
IF 7.014
Times Cited 5
Cellular slime molds G00691 S90286