| RRC ID |
6736
|
| 著者 |
Vourekas A, Kalavrizioti D, Zarkadis IK, Spyroulias GA, Stathopoulos C, Drainas D.
|
| タイトル |
A 40.7 kDa Rpp30/Rpp1 homologue is a protein subunit of Dictyostelium discoideum RNase P holoenzyme.
|
| ジャーナル |
Biochimie
|
| Abstract |
RNase P is an essential and ubiquitous endonuclease that mediates the maturation of the 5' ends of all precursor tRNA molecules. The holoenzyme from Dictyostelium discoideum possesses RNA and protein subunits essential for activity, but the exact composition of the ribonucleoprotein complex is still under investigation. Bioinformatic analysis of D. discoideum genome identified seven open reading frames encoding candidate RNase P protein subunits. The gene named drpp30 encodes a protein with a predicted molecular mass of 40.7 kDa that clusters with Rpp1 and Rpp30 RNase P protein subunits from Saccharomyces cerevisiae and human respectively, which have significantly lower molecular masses. Cloning and heterologous expression of DRpp30 followed by immunochemical analysis of RNase P active fractions demonstrates its association with RNase P holoenzyme. Furthermore, we show that DRpp30 can bind D. discoideum RNase P RNA and tRNA transcripts in vitro, giving a first insight of its possible role in D. discoideum RNase P function. Homology modeling using as a template the archaeal Ph1887p, and molecular dynamics simulations of the modeled structure suggest that DRpp30 adopts a TIM-barrel fold.
|
| 巻・号 |
89(3)
|
| ページ |
301-10
|
| 公開日 |
2007-3-1
|
| DOI |
10.1016/j.biochi.2006.11.006
|
| PII |
S0300-9084(06)00291-4
|
| PMID |
17207566
|
| MeSH |
Amino Acid Sequence
Animals
Cloning, Molecular
Computer Simulation
DNA, Complementary / chemistry
DNA, Complementary / genetics
Dictyostelium / enzymology*
Dictyostelium / genetics
Electrophoretic Mobility Shift Assay
Holoenzymes / chemistry
Holoenzymes / genetics
Holoenzymes / metabolism*
Immunoprecipitation
Models, Molecular
Molecular Sequence Data
Molecular Weight
Protein Binding
Protein Structure, Secondary
Protein Subunits / chemistry
Protein Subunits / genetics
Protein Subunits / metabolism
Protozoan Proteins / chemistry
Protozoan Proteins / genetics
Protozoan Proteins / metabolism*
Ribonuclease P / chemistry
Ribonuclease P / genetics
Ribonuclease P / metabolism*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
|
| IF |
3.413
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
|
