| RRC ID |
67379
|
| 著者 |
van de Schootbrugge C, van Asten F, Nagtegaal ID, Versleijen-Jonkers YM, van Laarhoven HW, Roeffen MH, Sweep FC, Bussink J, Kaanders JH, Pruijn GJ, Boelens WC, Span PN.
|
| タイトル |
αB-crystallin expression is correlated with phospho-ERK1/2 expression in human breast cancer.
|
| ジャーナル |
Int J Biol Markers
|
| Abstract |
αB-crystallin is regarded as a biomarker for triple-negative and/or basal-like breast cancer. In normal breast cells, overexpression of αB-crystallin leads to neoplastic-like changes, which likely relate to enhanced expression of phosphorylated ERK1/2 (pERK1/2). In this study, we investigated whether αB-crystallin expression is correlated to pERK1/2 expression in breast cancer. In a balanced tissue microarray the expression of αB-crystallin and pERK1/2 kinase were determined immunohistochemically, together with the triple-negativity and basal-like markers CK5/6 and SMA and the signaling molecules pAKT, pmTOR, EGFR, and IGF-1R. αB-crystallin expression significantly correlated with triple negativity and basal-like markers CK5/6 and SMA (Pearson Chi-square test p=0.004, p=0.001, and p<0.001, respectively). A significant correlation was also observed with pERK1/2 expression (p=0.002). siRNA-mediated knockdown of αB-crystallin in the triple-negative breast cell line MDA-MB468 did not show an effect on pERK1/2 expression levels, indicating that lowering the level of αB-crystallin does not reduce pERK1/2 expression. Our results confirm that αB-crystallin can be used as a biomarker for triple-negative and/or basal-like breast cancer. The expression of αB-crystallin correlates with pERK1/2 expression in breast cancer tissue suggesting that therapies targeting αB-crystallin might be considered for treatment of triple-negative or basal-like breast cancer.
|
| 巻・号 |
28(4)
|
| ページ |
e365-70
|
| 公開日 |
2013-12-17
|
| DOI |
10.5301/jbm.5000032
|
| PII |
1F14C83E-606C-4A9F-8F68-4631DB68746D
|
| PMID |
23722303
|
| MeSH |
Biomarkers, Tumor / biosynthesis
Cell Line, Tumor
Cohort Studies
Female
Gene Knockdown Techniques
Humans
Immunohistochemistry
Mitogen-Activated Protein Kinase 1 / biosynthesis*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / biosynthesis*
Mitogen-Activated Protein Kinase 3 / metabolism
Phosphorylation
Triple Negative Breast Neoplasms / enzymology
Triple Negative Breast Neoplasms / metabolism*
alpha-Crystallin B Chain / biosynthesis*
alpha-Crystallin B Chain / genetics
|
| リソース情報 |
| ヒト・動物細胞 |
anti-αB-crystallin(RCB1304) |
