RRC ID 67468
Author Iguchi Y, Ishihara S, Uchida Y, Tajima K, Mizutani T, Kawabata K, Haga H.
Title Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices.
Journal Cell Struct Funct
Abstract Numerous types of cancer cells migrate into extracellular tissues. This phenomenon is termed invasion, and is associated with poor prognosis in cancer patients. In this study, we demonstrated that filamin B (FLNb), an actin-binding protein, is highly expressed in cancer cell lines that exhibit high invasiveness, with a spindle morphology, into 3D collagen matrices. In addition, we determined that knockdown of FLNb in invasive cancer cells converts cell morphology from spindle-shaped, which is associated with high invasiveness, to round-shaped with low invasiveness. Furthermore, di-phosphorylation of myosin regulatory light chain (MRLC) and phosphorylation of focal adhesion kinase (FAK) are inhibited in FLNb-knockdown cancer cells. These results suggest that FLNb enhances invasion of cancer cells through phosphorylation of MRLC and FAK. Therefore, FLNb may be a new therapeutic target for invasive cancers.
Volume 40(2)
Pages 61-7
Published 2015-1-1
DOI 10.1247/csf.15001
PMID 25925610
MeSH Cell Adhesion Cell Culture Techniques Cell Line, Tumor Cell Movement Collagen / chemistry Epithelial Cells / metabolism* Epithelial Cells / pathology Fibroblasts / metabolism* Fibroblasts / pathology Filamins / antagonists & inhibitors Filamins / genetics* Filamins / metabolism Focal Adhesion Kinase 1 / genetics* Focal Adhesion Kinase 1 / metabolism Gene Expression Regulation, Neoplastic* Humans Myosin Light Chains / genetics* Myosin Light Chains / metabolism Phosphorylation RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Signal Transduction
IF 2.429
Human and Animal Cells A549