RRC ID 67535
著者 Boo K, Bhin J, Jeon Y, Kim J, Shin HJ, Park JE, Kim K, Kim CR, Jang H, Kim IH, Kim VN, Hwang D, Lee H, Baek SH.
タイトル Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
ジャーナル Nat Commun
Abstract The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination.
巻・号 6
ページ 6810
公開日 2015-4-10
DOI 10.1038/ncomms7810
PII ncomms7810
PMID 25857206
PMC PMC4403444
MeSH Animals Cell Differentiation Chromatin / chemistry Chromatin / metabolism Chromatin Assembly and Disassembly DNA Helicases / deficiency DNA Helicases / genetics* Epigenesis, Genetic* Gene Expression Profiling Genome-Wide Association Study Mice Mice, Knockout Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / metabolism* Octamer Transcription Factor-3 / deficiency Octamer Transcription Factor-3 / genetics* Patched Receptors Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism RNA, Long Noncoding / genetics* RNA, Long Noncoding / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism Tissue Inhibitor of Metalloproteinase-2 / genetics Tissue Inhibitor of Metalloproteinase-2 / metabolism Tropomyosin / genetics Tropomyosin / metabolism
IF 12.121
リソース情報
ヒト・動物細胞 ZHBTc4(AES0136)