RRC ID 67569
著者 Araki T, Osaka J, Kato Y, Shimozono M, Kawamura H, Iwanaga R, Hakeda-Suzuki S, Suzuki T.
タイトル Systematic identification of genes regulating synaptic remodeling in the Drosophila visual system.
ジャーナル Genes Genet Syst
Abstract In many animals, neural activity contributes to the adaptive refinement of synaptic properties, such as firing frequency and the number of synapses, for learning, memorizing and adapting for survival. However, the molecular mechanisms underlying such activity-dependent synaptic remodeling remain largely unknown. In the synapses of Drosophila melanogaster, the presynaptic active zone (AZ) forms a T-shaped presynaptic density comprising AZ proteins, including Bruchpilot (Brp). In a previous study, we found that the signal from a fusion protein molecular marker consisting of Brp and mCherry becomes diffuse under continuous light over three days (LL), reflecting disassembly of the AZ, while remaining punctate under continuous darkness. To identify the molecular players controlling this synaptic remodeling, we used the fusion protein molecular marker and performed RNAi screening against 208 neuron-related transmembrane genes that are highly expressed in the Drosophila visual system. Second analyses using the STaR (synaptic tagging with recombination) technique, which showed a decrease in synapse number under the LL condition, and subsequent mutant and overexpression analysis confirmed that five genes are involved in the activity-dependent AZ disassembly. This work demonstrates the feasibility of identifying genes involved in activity-dependent synaptic remodeling in Drosophila, and also provides unexpected insight into the molecular mechanisms involved in cholesterol metabolism and biosynthesis of the insect molting hormone ecdysone.
巻・号 95(3)
ページ 101-110
公開日 2020-8-27
DOI 10.1266/ggs.19-00066
PMID 32493879
MeSH Animals Cloning, Molecular / methods* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster Luminescent Proteins / genetics Luminescent Proteins / metabolism Neurons / metabolism RNA Interference Recombinant Proteins / genetics Recombinant Proteins / metabolism Synapses / metabolism* Synapses / physiology Visual Pathways / cytology Visual Pathways / metabolism*
IF 0.917
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