Reference - Detail
| RRC ID | 67728 |
|---|---|
| Author | Kishimoto M, Uemura K, Sanaki T, Sato A, Hall WW, Kariwa H, Orba Y, Sawa H, Sasaki M. |
| Title | TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. |
| Journal | Viruses |
| Abstract |
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development. |
| Volume | 13(3) |
| Published | 2021-2-28 |
| DOI | 10.3390/v13030384 |
| PII | v13030384 |
| PMID | 33671076 |
| PMC | PMC8001073 |
| MeSH | Angiotensin-Converting Enzyme 2 / metabolism Animals COVID-19 / metabolism* COVID-19 / virology* Chlorocebus aethiops HEK293 Cells Humans Membrane Proteins / metabolism* SARS-CoV-2 / metabolism* Serine Endopeptidases / metabolism* Serine Proteases / metabolism* Spike Glycoprotein, Coronavirus / metabolism* Vero Cells Virus Internalization |
| IF | 3.816 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 42 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CSII-CMV-MCS-IRES2-Bsd (RDB04385) CSII-CMV-TMPRSS11D-IRES2-Bsd (RDB19555) CSII-CMV-TMPRSS11E-IRES2-Bsd (RDB19556) CSII-CMV-TMPRSS13-IRES2-Bsd (RDB19557) |