| RRC ID |
67841
|
| Author |
Maeda M, Suzuki M, Takashima S, Sasaki T, Oh-Hashi K, Takemori H.
|
| Title |
The new live imagers MitoMM1/2 for mitochondrial visualization.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Mitochondria are eukaryotic organelles that consist of outer and inner bilayer membranes with a positive potential (H+) in the intermembrane space. This organelle plays an important role in ATP production and apoptosis. To observe the mitochondria in living cells, several fluorescent dyes (such as MitoTracker® [a standard mitochondrial imager] or rhodamine 123) have been developed. However, these reagents are unstable and exhibit a wide range of emission spectra, thereby hampering double staining results. Using recombinant DNA techniques, green or red fluorescent protein (GFP or RFP)-tagged proteins are now available for multi-color labeling of mitochondria. Here, we have discussed the development of the novel mitochondrial live imagers MitoMM1/2, derivatives of ATTO565; furthermore, MitoMM1/2 are sensitive to the membrane potential, resistant to detergents, and the fluorescence of MitoMM1/2 does not overlap with green fluorescence.
|
| Volume |
562
|
| Pages |
50-54
|
| Published |
2021-7-12
|
| DOI |
10.1016/j.bbrc.2021.05.040
|
| PII |
S0006-291X(21)00811-1
|
| PMID |
34034093
|
| MeSH |
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism*
Green Fluorescent Proteins / metabolism
HEK293 Cells
Humans
Membrane Potential, Mitochondrial
Mitochondria / metabolism*
Mitophagy
Molecular Imaging*
Tetraspanin 30 / metabolism
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
B16F10(RCB2630) |
