Reference - Detail
| RRC ID | 67841 |
|---|---|
| Author | Maeda M, Suzuki M, Takashima S, Sasaki T, Oh-Hashi K, Takemori H. |
| Title | The new live imagers MitoMM1/2 for mitochondrial visualization. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Mitochondria are eukaryotic organelles that consist of outer and inner bilayer membranes with a positive potential (H+) in the intermembrane space. This organelle plays an important role in ATP production and apoptosis. To observe the mitochondria in living cells, several fluorescent dyes (such as MitoTracker® [a standard mitochondrial imager] or rhodamine 123) have been developed. However, these reagents are unstable and exhibit a wide range of emission spectra, thereby hampering double staining results. Using recombinant DNA techniques, green or red fluorescent protein (GFP or RFP)-tagged proteins are now available for multi-color labeling of mitochondria. Here, we have discussed the development of the novel mitochondrial live imagers MitoMM1/2, derivatives of ATTO565; furthermore, MitoMM1/2 are sensitive to the membrane potential, resistant to detergents, and the fluorescence of MitoMM1/2 does not overlap with green fluorescence. |
| Volume | 562 |
| Pages | 50-54 |
| Published | 2021-7-12 |
| DOI | 10.1016/j.bbrc.2021.05.040 |
| PII | S0006-291X(21)00811-1 |
| PMID | 34034093 |
| MeSH | Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism* Green Fluorescent Proteins / metabolism HEK293 Cells Humans Membrane Potential, Mitochondrial Mitochondria / metabolism* Mitophagy Molecular Imaging* Tetraspanin 30 / metabolism |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | B16F10(RCB2630) |